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Products_type
Secondary Antibody
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Products_short_name
[IgE (epsilon chain)]
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Products_name_syn
[Goat anti-Human IgE (epsilon chain) - Affinity Pure]
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Other_names
[immunoglobulin E heavy chain, partial]
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Reactivity
Human. Cross Reactivity: Based on IEP, no reactivity is observed to: non-immunoglobulin human serum proteins light chains on all human immunoglobulins serum proteins from bovine, mouse or rabbit
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Specificity
Based on IEP, this antibody reacts with: heavy chains on Human IgE
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Purity
Affinity purified antibody is > 95% based on SDS-PAGE Affinity purified using solid phase Human IgE.
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Form
Clear, colorless liquid
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Concentration
1.50 mg/ml (E 1% at 280 nm = 13.0)
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Storage_stability
Store at 2-8 degree C. Shelf Life: 1 year from date of receipt. Prepare working dilution prior to use and then discard.
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Tested_application
ELISA (EIA), Immunohistochemistry (IHC)
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Test
A high affinity purification column was use to purify Goat anti- IgE (epsilon chain) - Pure, Conjugate, min x w/ , mouse rabbit serum proteins by MyBioSource by chromatographic size exclusion. MyBioSource supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. The ultrapure Goat anti- IgE (epsilon chain) - Pure, Conjugate, min x w/ , mouse rabbit serum proteins supplied from MyBioSource is supplied in 1. The reagent contains very low traces of production synthesis impurities and is highly purified by ultra filtration and distillation for the chemical compounds. Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.
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Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples. Immunoglobulin E (IgE) kappa or Fc specific antibody is a kind of antibody (or immunoglobulin (Ig) "isotype") that has only been found in mammals. IgE is synthesized by plasma cells. Monomers of IgE consist of two heavy chains (ε chain) and two light chains, with the ε chain containing 4 Ig-like constant domains (Cε1-Cε4). IgEs play a role in allergy and response to parasite infection. High levels of IgEs caused by parasites can lower the allergic reaction of patients to allergens common present in the human environment. As such the parasite history of an allergic patient needs to be taken in consideration as a positive factor.
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Conjugation
Biotinylated
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Latin name
Capra aegagrus hircus, Mus musculus, Oryctolagus cuniculus
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About
Rabbits are used for polyclonal antibody production by MyBioSource. Rabbit antibodies are very stable and can be stored for several days at room temperature. MyBioSource adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Serum should be stored at +5°C
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Group
Sera
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Gene target
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Short name
Goat anti- IgE (epsilon chain) - Pure, Conjugate, min x w/ , mouse rabbit serum proteins
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Technique
anti-Human, affinity, Rabbit, anti-, Mouse, serum, anti, Goat, Pure, IgE, antibody to, antibodies, antibodies against human proteins, goats, IgEs, mouses, pure products
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Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial. Rabbits
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Isotype
IgE, IgE
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Label
Biotin
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Species
Mouse, Bos Taurus genes are available form the Bovine Genome database. Goats, Humans, Mouses
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Alternative name
caprine Antibody toHuman immunoglobuline E (epsilon epitope) - protein Pure, biotinilated Conjugate, min x w/bovine, mouse and production species: rabbit blood serum proteins
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Alternative technique
rabbit-anti, sera, murine, antibodies, purified, caprine, immunoglobulin-e
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Tissue
serum
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