Donkey anti-Rabbit IgG (H&L) - Affinity Pure, FITC Conjugate, min x w/mouse IgG or serum proteins[IgG (H&L)]

• Catalog number
  MBS687585
• Price
  Please ask
• Size
  NA

• Products_type
  Secondary Antibody
• Reactivity
  Rabbit.Cross Reactivity: Based on IEP, no reactivity is observed to:non-immunoglobulin rabbit serum proteinsmouse IgG or serum proteins
• Test
  A high affinity purification column was use to purify anti-Rabbit IgG (H L) - Pure, FITC Conjugate, min x w/mouse IgG serum proteins[IgG (H L)] by MyBioSource by chromatographic size exclusion. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. The ultrapure anti-Rabbit IgG (H L) - Pure, FITC Conjugate, min x w/mouse IgG serum proteins[IgG (H L)] supplied from MyBioSource is supplied in 1. The reagent contains very low traces of production synthesis impurities and is highly purified by ultra filtration and distillation for the chemical compounds. Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.
• Latin name
  Equus africanus asinus, Mus musculus, Oryctolagus cuniculus
• Properties
  This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• About
  Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rabbits are used for polyclonal antibody production by MyBioSource. Rabbit antibodies are very stable and can be stored for several days at room temperature. MyBioSource adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Serum should be stored at +5°C
• Group
  Sera

• Gene target
  Conjugate   min   proteins[  
• Short name
  anti-Rabbit IgG (H L) - Pure, FITC Conjugate, min x w/mouse IgG serum proteins[IgG (H L)]
• Technique
  affinity, Rabbit, anti-, Mouse, serum, anti, FITC, Pure, IgG, antibody to, antibodies, Fluorescein, IgGs, mouses, pure products
• Host
  Donkey, Donkeys or Equus africanus asinus are often used as host species to raise antibodies. Even Equus ferus or horse antibodies are different than donkey antibodies since we know horse anti donkey IgGs. For immunohistochemistry it is useful to use an anti-donkey or horse block if your primary antibody is a donkey anti-human. We provide also anti-donkey as a species antibodies and ELISA kits. Rabbits
• Isotype
  IgG, Heavy & Light chains, IgG
• Label
  FITC
• Species
  Mouse, Donkeys, Mouses
• Alternative name
  Donkey Antibody tohost: rabbit Immunoglobulin G (heavy + light chain) - protein Pure, fluorecein Conjugate, min x w/mouse Immunoglobulin G and blood serum proteins[Immunoglobulin G (heavy + light chain)]
• Alternative technique
  rabbit-anti, sera, murine, antibodies, purified, fluorescine, immunoglobulins
• Tissue
  serum

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

Similar products