DNA Ligase I Rabbit Polyclonal

Products_type

Antibody
Products_short_name

[DNA Ligase I]
Other_names

[DNA ligase 1; DNA ligase 1; DNA ligase 1; DNA ligase 1; DNA ligase I; Polydeoxyribonucleotide synthase [ATP] 1]
Products_gene_name

[DNA]
Other_gene_names

[Lig1; Lig1]
Clonality

Polyclonal
Reactivity

Human, Mouse, Rat
Purity

>=95% as determined by SDS-PAGE Immunogen Affinity Purified
Form

Liquid
Storage_stability

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)
Tested_application

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Group

Polyclonals and antibodies
About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by MyBioSource. Rabbit antibodies are very stable and can be stored for several days at room temperature. MyBioSource adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Latin name

Oryctolagus cuniculus

Gene target

DNA Ligase
Gene symbol

PRKDC, HLA-DOA
Short name

DNA Ligase I Rabbit Polyclonal
Technique

Polyclonal, Rabbit, dna, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
Host

Rabbit, Rabbits
Isotype

IgG
Alternative name

Desoxyribonucleic acid Ligase I production species: rabbit polyclonal
Alternative technique

polyclonals, rabbit-anti

Name

Ligase Chain Reaction
Concept

Scope note: A DNA amplification technique based upon the ligation of OLIGONUCLEOTIDE PROBES. The probes are designed to exactly match two adjacent sequences of a specific target DNA. The chain reaction is repeated in three steps in the presence of excess probe: (1) heat denaturation of double-stranded DNA, (2) annealing of probes to target DNA, and (3) joining of the probes by thermostable DNA ligase. After the reaction is repeated for 20-30 cycles the production of ligated probe is measured.
Tree numbers
- E05.393.620.311
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data