CD4 FITC Monoclonal Antibody (Clone RPA-T4)
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Catalog number
MBS840058
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Price
Please ask
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Size
25 Tests
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Products_type
Antibody
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Products_short_name
[CD4]
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Products_name_syn
[CD4]
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Other_names
[T-cell surface glycoprotein CD4 isoform 1; T-cell surface glycoprotein CD4; T-cell surface glycoprotein CD4; CD4 molecule; T-cell surface antigen T4/Leu-3; CD_antigen: CD4]
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Products_gene_name
[CD4]
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Other_gene_names
[CD4; CD4; CD4mut]
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Clonality
Monoclonal
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Clone
[RPA-T4]
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Reactivity
Human
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Purity
Affinity chromatography.
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Form
Phosphate-buffered aqueous solution pH 7.2,
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Storage_stability
4 degree C Shelf Life: 12 months
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Tested_application
Flow Cytometry (FC/FACS)
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Properties
If you buy Antibodies supplied by MyBioSource they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
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Description
CD4+ T helper cell marker or cluster differentiator of white blood cells in FACS or flow cytometry. Used offer coupled to magnetic beads. GENTAUR makes custom magnetic bead couplings on 50 and 250 nm beads for certain clones.
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Conjugation
Anti-FITC Antibody
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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French translation
anticorps
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Gene target
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Gene symbol
CD4, B3GNT4, B4GALT4, GALNT4, B3GALT4, SERPINB3
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Short name
CD4 FITC Monoclonal Antibody (Clone RPA-T4)
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Technique
Antibody, FITC, antibodies against human proteins, antibodies for, Fluorescein, Monoclonals or monoclonal antibodies
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Host
Mouse
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Isotype
IgG1, Kappa
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Label
FITC
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Alternative name
CD4 molecule fluorecein monoclonal (antibody to-) (clonality RPA-T4)
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Alternative technique
antibodies, fluorescine
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Alternative to gene target
CD4 molecule, CD4mut, CD4 and IDBG-15034 and ENSG00000010610 and 920, protein homodimerization activity, Cell surfaces, Cd4 and IDBG-188351 and ENSMUSG00000023274 and 12504, CD4 and IDBG-640247 and ENSBTAG00000003255 and 407098
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Clone name
RPA-T4
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Gene info
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Identity
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Gene
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Long gene name
CD4 molecule
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Synonyms gene name
- CD4 antigen (p55)
- T-cell surface glycoprotein CD4
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GenBank acession
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Locus
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Discovery year
1986-01-01
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Entrez gene record
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RefSeq identity
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Classification
- CD molecules
- C2-set domain containing
- V-set domain containing
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VEGA ID
Gene info
Gene info
Gene info
Gene info
Gene info
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Identity
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Gene
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Long gene name
serpin family B member 3
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Synonyms gene
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Synonyms gene name
- squamous cell carcinoma antigen 1
- serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 3
- serpin peptidase inhibitor, clade B (ovalbumin), member 3
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Synonyms
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GenBank acession
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Locus
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Discovery year
1995-08-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Serpin peptidase inhibitors
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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