CD4 antibody FITC
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Catalog number61R-CD4gHUFT
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PricePlease ask
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Size120 tests
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CategoryPrimary Antibody
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Antibody SubtypeConjugated Monoclonal Antibodies
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Area of researchImmunology
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Type of ImmunogenCD4 antibody (FITC) was raised in mouse using P815 cell transfected with human CD4 ..
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Raised inMouse
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SpecificityHuman
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Cross ReactivityShould be determined by the end user
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IsotypesIgG2
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CloneM-T441
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Conjugate TagFITC
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Method of PurificationCD4 antibody (FITC) was purified by Protein A chromatography.
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Concentration0.25 mg/ml
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Form Buffer50 mM Na3PO4, pH 7.5, with 100 mM KCL, 150 mM NaCl, 5% Glycerol, 0.2% BSA, and 0.04% NaN3.
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StorageStore at 4 deg C. Keep away from light.
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Shipping conditionsBlue Ice
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Tested forELISA; FC
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Usage RecommendationsFC: 10 ug/ml
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Additional InformationThis antibody recognizes the domain 2 region of the CD4 molecule and reacts with human CD4 cell transfectants.
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PropertiesIf you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.
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DescriptionCD4+ T helper cell marker or cluster differentiator of white blood cells in FACS or flow cytometry. Used offer coupled to magnetic beads. GENTAUR makes custom magnetic bead couplings on 50 and 250 nm beads for certain clones.
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ConjugationAnti-FITC Antibody
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French translationanticorps
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Gene target
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Gene symbolCD4
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Short nameCD4 antibody FITC
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TechniqueAntibody, FITC, antibodies against human proteins, antibodies for, Fluorescein
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LabelFITC
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Alternative nameMouse monoclonal CD4 antibody (FITC)
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Alternative techniqueantibodies, fluorescine
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Alternative to gene targetCD4 molecule, CD4mut, CD4 and IDBG-15034 and ENSG00000010610 and 920, protein homodimerization activity, Cell surfaces, Cd4 and IDBG-188351 and ENSMUSG00000023274 and 12504, CD4 and IDBG-640247 and ENSBTAG00000003255 and 407098
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Gene info
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Identity
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Gene
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Long gene nameCD4 molecule
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Synonyms gene name
- CD4 antigen (p55)
- T-cell surface glycoprotein CD4
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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RefSeq identity
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Classification
- CD molecules
- C2-set domain containing
- V-set domain containing
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data