Biotin Mouse Anti-Goat IgG Monoclonal Antibody
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Catalog number
MBS154170
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Price
Please ask
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Size
0.05 mg
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Products_type
Secondary Antibody
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Products_short_name
[IgG]
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Reactivity
Goat. No cross reactivity with immunoglobulins from other species.
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Purity
The purified antibody-Biotin conjugate is separated from non-conjugated biotin by dialysis.
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Form
Liquid
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Concentration
1 mg/mL
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Storage_stability
Store at 2-8 degree C. The product is stable for about 4-6 weeks at 4 degree C as an undiluted liquid. For extended storage, aliquot and freeze the product at -70 degree C or below. Avoid repeated freezing and thawing. Or alternatively, add an equal volume of glycerol for a final concentration of 50% and store at -20 degree C.
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Tested_application
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by MyBioSource they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Conjugation
Biotinylated
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Latin name
Capra aegagrus hircus, Mus musculus
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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French translation
anticorps
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Gene target
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Short name
Mouse Anti-Goat IgG Monoclonal Antibody
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Technique
Antibody, anti-, Mouse, anti, Goat, IgG, antibody to, antibodies, antibodies against human proteins, antibodies for, goats, IgGs, Monoclonals or monoclonal antibodies, mouses
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Host
Mouse, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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Isotype
IgG2, IgG
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Label
Biotin
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Species
Mouse, Goats, Mouses
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Alternative name
biotinilated Mouse Antibody toGoat Immunoglobulin G monoclonal (antibody to-)
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Alternative technique
antibodies, murine, caprine, immunoglobulins
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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