Goat anti Human IgG biotin

  • Catalog number
    43R-1187
  • Price
    Please ask
  • Size
    2 mg
  • Category
    Secondary Antibody
  • Antibody Subtype
    Conjugated Secondary Antibodies
  • Area of research
    Immunology
  • Type of Immunogen
    Purified Human IgG,
  • Raised in
    Goat
  • Specificity
    This antibody reacts with heavy chains on human IgG (gamma chain).
  • Cross Reactivity
    No reactivity is observed to non-immunoglobulin human serum proteins or light chains on all human immunoglobulins or human IgA + IgM.
  • Conjugate Tag
    Biotin
  • Grade Purity
    Grater than 95% pure
  • Method of Purification
    Affinity purified using solid phase Human IgG (H&L)
  • Source
    Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
  • Concentration
    1.0 mg/ml (lot specific)
  • Form Buffer
    Clear, colorless liquid, containing 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, 1% (w/v) BSA and 0.05% (w/v) NaN3. Protease/IgG free, pH 7.2.
  • Storage
    Store at 2-8 deg C.
  • Shipping conditions
    Blue Ice
  • Tested for
    Please refer to the datasheet
  • Usage Recommendations
    1:2000-1:20,000 ELISA/WB, 1:500-1:5000 IHC
  • URL
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
  • Properties
    Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Conjugation
    Biotinylated
  • Latin name
    Capra aegagrus hircus
  • About
    Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
  • Short name
    Goat anti IgG
  • Technique
    anti human, anti, Goat, IgG, antibody to, anti human antibodies, goats, IgGs
  • Host
    Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
  • Isotype
    IgG, IgG
  • Label
    Biotin
  • Species
    Human, Goats, Humans
  • Alternative name
    Goat anti Human IgG secondary antibody (biotin)
  • Alternative technique
    antibodies, caprine, immunoglobulins
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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