Anti-Mouse I-Ab (public; Ia.m8), FITC (Clone 28-16-8S) (mouse IgM)
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Catalog numberMBS520349
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Price370.98 USD
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Size0.1 mg
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Products_typeAntibody
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Products_short_name[I-Ab (public; Ia.m8)]
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Products_name_syn[I-Ab (public; Ia.m8), FITC (Clone 28-16-8S) (mouse IgM); FITC Anti-Mouse I-Ab Monoclonal Antibody]
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ClonalityMonoclonal
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Clone[28-16-8S]
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ReactivityMouse
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SpecificityI-Ab (Mouse MHC class II I-Ab, cross reacts with I-Ad)
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FormFITC
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ConcentrationAntibody Concentration: 0.1 mg/ml
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Storage_stabilityStore at 4 degree C. For long term storage, aliquot and freeze unused portion at -20 degree C in volumes appropriate for single usage. Avoid freeze/thaw cycles and prolonged exposure to light. If the reagent is being diluted, it is recommended that only the quantity to be used within one week be diluted. Check label for expiry date.
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Tested_applicationImmunohistochemistry (IHC)
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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PropertiesThis MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes. Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
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ConjugationAnti-FITC Antibody
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Gene symbolIGHV4-28, SNORD114-28, RNVU1-28, SNORD116-28, SNORD115-28, ESB3, ERVW-28, ERVK-28, IGKV2-28
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Short nameAnti-Mouse I-Ab (public; Ia m8), FITC (Clone 28-16-8S) (mouse IgM)
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Techniqueanti-, Mouse, anti, FITC, IgM, antibody to, antibodies, Fluorescein, IgMs, mouses
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HostMouse
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IsotypeMouse IgM, IgM
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LabelFITC
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SpeciesMouse, Mouses
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Alternative nameAntibody toMouse I-Antibody (public; Ia.m8), fluorecein (clonality 28-16-8S) (mouse Immunoglobulin M)
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Alternative techniquemurine, antibodies, fluorescine, immunoglobulin-m
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Clone name28-16-8S
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Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 4-28
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 114-28
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Synonyms
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Locus
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Discovery year2006-09-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameRNA, variant U1 small nuclear 28
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Locus
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Discovery year2019-07-26
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Classification
- Variant U1 small nuclear RNAs
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 116-28
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 115-28
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Synonyms
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Locus
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Discovery year2011-08-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameesterase B3
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Locus
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Discovery year2001-06-22
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Pubmed identfication
Gene info
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Identity
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Gene
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Long gene nameendogenous retrovirus group W member 28
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Synonyms gene name
- endogenous retrovirus group W, member 28
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GenBank acession
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Locus
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Discovery year2013-10-21
Gene info
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Identity
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Gene
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Long gene nameendogenous retrovirus group K member 28
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Synonyms gene name
- endogenous retrovirus group K, member 28
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GenBank acession
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Locus
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Discovery year2014-01-03
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Pubmed identfication
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 2-28
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data