Anti-Mouse CD27 FITC (Clone LG.3A10) (Armenian Hamster IgG1)
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Catalog number
MBS520087
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Price
Please ask
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Size
0.1 mg
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Products_type
Antibody
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Products_short_name
[CD27]
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Products_name_syn
[CD27 FITC (Clone LG.3A10) (Armenian Hamster IgG1); Anti-Mouse CD27 Monoclonal Antibody]
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Other_names
[CD27 antigen; CD27 antigen; CD27 antigen; CD27L receptor; T cell activation antigen S152; T-cell activation antigen CD27; tumor necrosis factor receptor superfamily, member 7; CD27 molecule; CD27L receptor; T-cell activation antigen CD27; T14; Tumor necrosis factor receptor superfamily member 7]
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Products_gene_name
[CD27]
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Other_gene_names
[CD27; CD27; T14; S152; Tp55; TNFRSF7; S152. LPFS2; TNFRSF7]
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Clonality
Monoclonal
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Clone
[LG.3810]
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Reactivity
Mouse
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Specificity
CD27 (Mouse CD27 Receptor)
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Form
FITC
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Concentration
0.1 mg/ml
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Storage_stability
For all formats, store at 4 degree C. DO NOT FREEZE PE conjugates. For long term storage (Purified, Biotin, FITC, No Azide), aliquot and freeze unused portion at -20 degree C in volumes appropriate for single usage. Avoid freeze/thaw cycles.
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Tested_application
Immunohistochemistry (IHC), Flow Cytometry (FC/FACS) (stimulating)
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes. Armenian of Syrian hamster monoclonal IgGs are useful antibodies for double staining your IHC samples. Using mabs or monoclonal antibodies has a lot of advantages for Flow cytometry.
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Conjugation
Anti-FITC Antibody
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Gene target
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Gene symbol
CD27-AS1, CD27
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Short name
Anti-Mouse CD27 FITC (Clone LG 3A10) (Armenian IgG1)
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Technique
anti-, Mouse, anti, FITC, antibody to, antibodies, Fluorescein, mouses
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Host
Armenian Hamster
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Isotype
Armenian hamster IgG1, IgG1
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Label
FITC
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Species
Hamster, Hamsters, Mouses
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Alternative name
Antibody toMouse CD27 molecule fluorecein (clonality LG.3A10) (Armenian Hamster IgG1)
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Alternative technique
murine, antibodies, fluorescine
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Alternative to gene target
CD27 molecule, S152 and S152. LPFS2 and T14 and TNFRSF7 and Tp55, CD27 and IDBG-14014 and ENSG00000139193 and 939, cysteine-type endopeptidase inhibitor activity involved in apoptotic process, Extracellular, Cd27 and IDBG-189750 and ENSMUSG00000030336 and 21940, CD27 and IDBG-640494 and ENSBTAG00000014725 and 512514
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Clone name
LG
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Gene info
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Identity
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Gene
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Long gene name
CD27 antisense RNA 1
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Synonyms gene name
- CD27 antisense RNA 1 (non-protein coding)
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GenBank acession
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Locus
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Discovery year
2012-04-27
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Entrez gene record
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RefSeq identity
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Classification
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
CD27 molecule
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Synonyms gene
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Synonyms gene name
- tumor necrosis factor receptor superfamily, member 7
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Synonyms
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GenBank acession
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Locus
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Discovery year
1992-06-10
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Entrez gene record
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Pubmed identfication
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Classification
- Tumor necrosis factor receptor superfamily
- CD molecules
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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