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Armenian Hamster anti CD27

  • Catalog number
    MUB0365S
  • Product name
    Armenian Hamster anti CD27
  • Size
    1 mL
  • Category
    Primary Antibodies
  • Long description
    Members of the tumor necrosis factor receptor (TNFR) family are emerging as important molecules impliCated in the regulation of prolifeRation, differentiation and survival of T and B lymphocytes. Among these receptors is CD27, the function of which has thus far only been studied in the Human system, where it amplifies the T cell prolifeRative response induced by TCR triggering. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for geneRation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-κB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a pro-apoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.
  • Antibody come from
    mAbs to murine CD27 were geneRated injecting a mixture of the mCD27tag expresssing clones AR0524 and AR0530; Hamster spleen cells and SP2/0 murine myeloma cells were fused.
  • Other description
    Each vial contains 1ml of culture supernatant of monoclonal antibody containing 0.09% sodium azide.
  • Clone
    LG.3A10
  • Isotype
    IgG
  • Antigen antibody binding interaction
    Armenian Hamster anti CD27 Antibody
  • Antibody is raised in
    Armenian Hamster
  • Antibody s reacts with
    Human,Mouse
  • Antibody s reacts with these species
    This antibody doesn't cross react with other species
  • Label
    unlabeled
  • Antibody s specificity
    LG.3A10 and LG.8A6 see a different epitope. A combination can therefore be developed into a double determinant ELISA to detect soluble Mouse CD27. LG.3A10 cross-react with Human CD27, LG.8A6 does not.
  • Research interest
    Cell adhesion,Immunology
  • Application
    ELISA,Flow Cytometry,Immunohistochemistry (frozen),Immunohistochemistry (paraffin),Immunoprecipitation
  • Antibody s suited for
    LG.3A10 is useful for flow cytometry, immunoprecipitation and immunohistochemistry on frozen and paraffin embedded sections . Optimal antibody dilution should be determined by titration. Recommended dilution for immunohistochemistry: 1:100. In combination with LG.8A6, it is possible to develop a double determinant ELISA to detect soluble Mouse CD27.
  • Storage
    Store at 4°C, or in small aliquots at -20°C.
  • Relevant references
    Gravestein, L.A., Nieland, J.D., Kruisbeek, A.M. and Borst, J. Int. Immunol. 7: 551-557, 1995. Gravestein, L., van Ewijk, W., Ossendorp, F. and Borst, J. J. Exp. Med. 184: 675-685, 1996. Hendriks, J., Gravestein, L.A., Tesselaar, K., van Lier, R.A.W., Schumacher, T.N.M., and Borst, J.Nature Immunol. 1: 433-440, 2000.
  • Protein number
    P26842
  • Warnings
    This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
  • Antibody s link
    Antibody_s_link
  • Alternative to gene target
    • [ "CD27 molecule", "S152 and S152. LPFS2 and T14 and TNFRSF7 and Tp55", "CD27 and IDBG-14014 and ENSG00000139193 and 939", "cysteine-type endopeptidase inhibitor activity involved in apoptotic process", "Extracellular", "Cd27 and IDBG-189750 and ENSMUSG00000030336 and 21940", "CD27 and IDBG-640494 and ENSBTAG00000014725 and 512514" ]
  • Gene target
    Armenian CD27
    • Gene info
    Gene info
    Locus Specific Databases:LRG_357
  • Gene symbol
    CD27-AS1, CD27
  • Host
    Hamster
  • isotype filter
    • IgG
  • Short name
    Armenian anti CD27
  • label filter
    • unlabeled
  • technique filter
    • anti
  • Technique
    anti, antibody to
  • Alternative technique
    antibodies