Anti-Human CTLA-4, FITC, (clone: A3.4H2.H12), (mouse IgG2ak)

• Catalog number
  MBS520258
• Price
  Please ask
• Size
  0.1 mg

• Products_type
  Antibody
• Products_short_name
  [CTLA-4]
• Products_name_syn
  [CTLA-4, FITC, (clone: A3.4H2.H12), (mouse IgG2ak); FITC Anti-Human CTLA-4 (CD152) Monoclonal Antibody]
• Other_names
  [cytotoxic T-lymphocyte protein 4 isoform CTLA4-TM; Cytotoxic T-lymphocyte protein 4; cytotoxic T-lymphocyte protein 4; CD152 isoform; celiac disease 3; cytotoxic T-lymphocyte antigen 4; cytotoxic T-lymphocyte-associated antigen 4; cytotoxic T-lymphocyte-associated serine esterase-4; cytotoxic T lymphocyte associated antigen 4 short spliced form; ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4; cytotoxic T-lymphocyte-associated protein 4; Cytotoxic T-lymphocyte-associated antigen 4]
• Products_gene_name
  [CTLA-4]
• Other_gene_names
  [CTLA4; CTLA4; CD; GSE; GRD4; CD152; CTLA-4; IDDM12; CELIAC3; CD152; CTLA-4]
• Clonality
  Monoclonal
• Clone
  [A3.4H2.H12]
• Reactivity
  Human
• Specificity
  Human CTLA-4 (CD152)
• Form
  FITC conjugated IgG buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml.
• Concentration
  Antibody Concentration: 0.1 mg/ml
• Storage_stability
  For all formats, store at 4°C. For long term storage (Purified and FITC), aliquot and freeze unused portion at -20°C in volumes appropriate for single usage. Avoid freeze/thaw cycles.
• Tested_application
  Flow Cytometry (FC/FACS)
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Test
  MyBioSource supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Properties
  This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Conjugation
  Anti-FITC Antibody
• Latin name
  Mus musculus

• Gene target
  CTLA-4   4H2   H12   IgG2ak  
• Gene symbol
  CTLA4, MIR1302-4, PIRC18, PIRC16, SNORD114-4, MIR941-4, IGKV2OR2-4, ANKRD20A12P
• Short name
  Anti- CTLA-4, FITC, (clone: A3 4H2 H12), (mouse IgG2ak)
• Technique
  anti-Human, anti-, Mouse, anti, FITC, antibody to, antibodies, antibodies against human proteins, Fluorescein, mouses
• Host
  Mouse
• Isotype
  Mouse IgG2ak
• Label
  FITC
• Species
  Mouse, Humans, Mouses
• Alternative name
  Antibody toHuman CTLA-4, fluorecein, (clonality: A3.4H2.H12), (mouse IgG2ak)
• Alternative technique
  murine, antibodies, fluorescine

Gene info

• Identity
  HGNC:2505
• Gene
  CTLA4
• Long gene name
  cytotoxic T-lymphocyte associated protein 4
• Synonyms gene
  • CELIAC3
  • IDDM12
• Synonyms gene name
  • celiac disease 3
  • insulin-dependent diabetes mellitus 12
• Synonyms
  • CD152
  • CD
  • GSE
  • CTLA-4
• Locus
  2q33.2
• Discovery year
  1989-05-25
• Entrez gene record
  1493
• Pubmed identfication
  • 3220103
  • 8817351
• RefSeq identity
  • NM_005214
• Classification
  • V-set domain containing
  • CD molecules
• VEGA ID
  OTTHUMG00000132877
• Locus Specific Databases
  • LRG_1220

Gene info

• Identity
  HGNC:35296
• Gene
  MIR1302-4
• Long gene name
  microRNA 1302-4
• Synonyms gene
  • MIRN1302-4
• Synonyms
  • hsa-mir-1302-4
• Locus
  2q33.3
• Discovery year
  2008-11-03
• Entrez gene record
  100302130
• RefSeq identity
  • NR_031633
• Classification
  • MicroRNA MIR1302 family

Gene info

• Identity
  HGNC:37522
• Gene
  PIRC18
• Long gene name
  piwi-interacting RNA cluster 18
• Locus
  4
• Discovery year
  2009-11-05
• Entrez gene record
  100313906
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37520
• Gene
  PIRC16
• Long gene name
  piwi-interacting RNA cluster 16
• Locus
  4
• Discovery year
  2009-11-05
• Entrez gene record
  100313849
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:32992
• Gene
  SNORD114-4
• Long gene name
  small nucleolar RNA, C/D box 114-4
• Synonyms
  • 14q(II-4)
• Locus
  14q32.31
• Discovery year
  2006-09-26
• Entrez gene record
  767580
• Pubmed identfication
  • 12045206
• RefSeq identity
  • NR_003196.1
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:33687
• Gene
  MIR941-4
• Long gene name
  microRNA 941-4
• Synonyms gene
  • MIRN941-4
• Synonyms
  • hsa-mir-941-4
• Locus
  20q13.33
• Discovery year
  2007-10-24
• Entrez gene record
  100126330
• RefSeq identity
  • NR_040032
• Classification
  • MicroRNAs

Gene info

• Identity
  HGNC:5809
• Gene
  IGKV2OR2-4
• Long gene name
  immunoglobulin kappa variable 2/OR2-4 (pseudogene)
• Synonyms gene name
  • immunoglobulin kappa variable 2/OR2-4
  • immunoglobulin kappa variable 2/OR2-4 pseudogene
• Synonyms
  • IGKV2/OR2-4
• GenBank acession
  • X51883
• Locus
  2q11.2
• Discovery year
  2000-04-18
• Entrez gene record
  28858
• Pubmed identfication
  • 2111145
• Classification
  • Immunoglobulin kappa (IGK) orphons

Gene info

• Identity
  HGNC:43603
• Gene
  ANKRD20A12P
• Long gene name
  ankyrin repeat domain 20 family member A12, pseudogene
• Synonyms gene name
  • ankyrin repeat domain 20 family, member A12, pseudogene
• Locus
  4
• Discovery year
  2011-11-23
• Entrez gene record
  100874392

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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