Rat anti Rat anti-mouse IgG2ak or IgG2bk antibody conjugated to PE
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Catalog number
RG24
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Price
Please ask
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Size
100 Tests
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Category
Second Step Reagents
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Long description
Second Step reagents labeled with FITC or PE
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Antibody come from
n/a
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Other description
Provided as sterile filtered solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein. Protein A/G Chromatography
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Clone
not specified
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Antigen antibody binding interaction
Rat anti Rat anti-mouse IgG2ak or IgG2bk antibody conjugated to PE Antibody
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Antibody is raised in
Rat
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Antibody s reacts with
see techfile
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Flow Cytometry
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Antibody s suited for
10 µl of purified reagent MAB/100 µl of 10^6 PBMC(peripheral blood mononuclear cells) in PBS. Mix gently and incubate for 15 minutes at 2¼-8¼C. Wash twice with PBS and add either 10 µl of R M IgG2a or IgG2b PE or 10 µl of R M IgG2a or IgG2b FITC depending on the desired fluorescence and analyzed immediately. See instrument manufacturerÕs instructions for Immunofluorescence analysis with a flow cytometer or microscope.
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Storage
4ºC
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Relevant references
no information yet
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus, Rattus norvegicus
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About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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French translation
anticorps
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Gene target
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Short name
anti anti-mouse IgG2ak IgG2bk antibody conjugated PE
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Technique
Antibody, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, antibodies for, antibody Conjugates, mouses
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Host
Rat
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Isotype
not specified
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Label
PE
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Species
Mouse, Mouses, Rats
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Alternative name
Rat antibody to Rat Antibody tomouse IgG2ak and IgG2bk (antibody to-) coupled to peroxidase
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Alternative technique
antibodies, murine
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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