Alkaline Phosphatase Conjugated Affinity Purified anti-Rabbit IgG [H&L] [Goat]
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Catalog number
MBS417844
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Price
Please ask
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Size
0.25 mg
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Products_type
Secondary Antibody
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Products_short_name
[IgG [H&L]]
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Products_gene_name
[IgG]
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Reactivity
Rabbit
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Concentration
1.0 mg/ml (by UV absorbance at 280 nm)
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Storage_stability
Store vial at 4 degree C before opening. DO NOT FREEZE. This product is stable at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of opening product.
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Tested_application
Immunoblotting, Western Blot (WB), Dot Blot (DB), ELISA (EIA), Immunohistochemistry (IHC), Other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency
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Test
A high affinity purification column was use to purify Alkaline Phosphatase Conjugated anti-Rabbit IgG [H L] [Goat] by MyBioSource by chromatographic size exclusion.
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Description
1 of protein A or G purified by MyBioSource will give more specificity than crude serum. This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Latin name
Capra aegagrus hircus, Oryctolagus cuniculus
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rabbits are used for polyclonal antibody production by MyBioSource. Rabbit antibodies are very stable and can be stored for several days at room temperature. MyBioSource adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Gene target
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Short name
Alkaline Phosphatase Conjugated anti-Rabbit IgG [H L] [Goat]
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Technique
affinity, purified, Rabbit, anti-, anti, Goat, IgG, antibody to, antibodies, antibody Conjugates, goats, IgGs, purifications
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Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial. Rabbits
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Isotype
IgG, Heavy & Light chains, IgG
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Label
Alkaline Phosphatase
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Species
Goats
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Alternative name
Alkaline Phosphatase coupled protein enriched Antibody tohost: rabbit Immunoglobulin G [heavy + light chain] [caprine]
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Alternative technique
pure, rabbit-anti, antibodies, caprine, immunoglobulins
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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