Alkaline Phosphatase Conjugated Affinity Purified anti-Sheep IgG [H&L] [Donkey]
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Catalog number
MBS417847
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Price
Please ask
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Size
0.25 mg
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Products_type
Secondary Antibody
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Products_short_name
[IgG [H&L]]
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Products_gene_name
[IgG]
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Reactivity
Sheep
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Concentration
1.0 mg/ml (by UV absorbance at 280 nm)
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Storage_stability
Store vial at 4 degree C before opening. DO NOT FREEZE. This product is stable at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of opening product.
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Tested_application
Immunoblotting, Western Blot (WB), Dot Blot (DB), ELISA (EIA), Immunohistochemistry (IHC), Other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency
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Test
A high affinity purification column was use to purify Alkaline Phosphatase Conjugated anti- IgG [H L] [ ] by MyBioSource by chromatographic size exclusion.
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Description
1 of protein A or G purified by MyBioSource will give more specificity than crude serum. This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Latin name
Equus africanus asinus
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
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Gene target
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Short name
Alkaline Phosphatase Conjugated anti- IgG [H L] [ ]
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Technique
affinity, purified, anti-, anti, IgG, antibody to, antibodies, antibody Conjugates, IgGs, purifications
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Host
Donkey, Donkeys or Equus africanus asinus are often used as host species to raise antibodies. Even Equus ferus or horse antibodies are different than donkey antibodies since we know horse anti donkey IgGs. For immunohistochemistry it is useful to use an anti-donkey or horse block if your primary antibody is a donkey anti-human. We provide also anti-donkey as a species antibodies and ELISA kits.
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Isotype
IgG, Heavy & Light chains, IgG
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Label
Alkaline Phosphatase
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Species
Sheep, Donkeys, The sheep (Ovis aries) ELISAs are detecting sheep proteins and antigens. Some epitopes will also recognize goat proteins. Sheep and goats are also used to produce polyclonals. An adult female sheep is referred to as a ewe a male as a ram, a castrated male as a wether, and a younger sheep as a lamb. Sheeps
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Alternative name
Alkaline Phosphatase coupled protein enriched Antibody toSheep Immunoglobulin G [heavy + light chain] [Donkey]
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Alternative technique
pure, antibodies, immunoglobulins
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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