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Alternative name1
Anti-SHEEP ANTI PIG AMYLASE (PANCREATIC):FITC
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Alternative name2
Ovine ANTI PIG AMYLASE (PANCREATIC):FITC
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Alternative name3
Ovinae ANTI PIG AMYLASE (PANCREATIC):FITC
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Alternative name4
AMYLASE
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Alternative name5
N/A
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Other names
pancreatic alpha-amylase; Pancreatic alpha-amylase; pancreatic alpha-amylase; PA; 1,4-alpha-D-glucan glucanohydrolase; N/A; 1,4-alpha-D-glucan glucanohydrolase
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Gene name
N/A
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Gene name synonims
N/A
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Other gene names
AMY2; AMY2; AMY2B; PA
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Category
Antibodies
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Clonality
Polyclonal
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Immunoglobulin isotype
Polyclonal IgG
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Clone
Polyclonal antibody
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Host organism
Sheep
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Species reactivity
Pig
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Specificity and cross reactivity
AMYLASE This item recognizes Amylase (Pancreatic), a digestive enzyme with a molecular weight of approximately 56 kDa secreted by the pancreas. _x000D__x000D_Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreatic gland. _x000D__x000D_This item reacts with both porcine and human pancreatic amylase.
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Purification method
N/A
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Form Appearance
FITC (Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid)
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Concentration
IgG concentration 1.0mg/ml
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Storage and shipping
Store at +Store productone at +4 degrees Celsius. or productone should be stored at -20 degrees Celsius. if preferred. Storage in frost-free freezers is not recommended. productone should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should productone contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of dispatch.
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Tested for
Immunofluorescence (IF), ELISA (EIA)
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Description
productone is a polyclonal antibody of high purity and binding affinity for the antigen that it is risen against. Properly used, this antibody will ensure excellent and reproducible results with guaranteed success for the applications that it is tested in. Polyclonal antibodies have series of advantages - larger batches can be supplied at a time, they are inexpensive to manufacture and respectively to buy, the time needed for production is considerably shorter. Polyclonal antibodies generally are more stable and retain their reactivity under unfavorable conditions. To obtain more detailed information on productone, please, refer to the full product datasheet.
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Advisory
In order to retain the quality and the affinity of productone unchanged, please, avoid cycles of freezing and thawing. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
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Additional description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Properties
If you buy Antibodies supplied by MBS Polyclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This MBS Polyclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Polyclonals FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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About
Pigs and the smaller guinea pigs are frequent used as models for humans.
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French translation
anticorps
