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Category
Primary Antibodies
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Long description
The reactivity of the antiserum is restricted to salivary amylase. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against human saliva a single precipitin line is obtained and shows a reaction of full identity with the purified immunogen. No reaction is obtained with human plasma. In precipitating techniques as immunoelectrophoresis and single and double radial immunodiffusion (Mancini, Ouchterlony) to identify the presence of salivary amylase or to determine its concentration. The presence of non-precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in non-precipitating antibodybinding techniques if proper controls are included.
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Antibody come from
Human salivary amylase is an enzyme hydrolyzing the alpha-1,4-glucan links in polysaccharides. Its molecular weight is 55,000. The immunogen has been isolated from pooled saliva and purified to homogeneity for use in the antiserum production. Freund’s complete adjuvant is used in the first step of the immunization procedure.
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Other description
Delipidated, heat inactivated, lyophilized, stable antiserum. No preservative added Total protein and IgG concentration are comparable to those of pooled normal sheep serum. No foreign proteins added
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Clone
Polyclonal
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Antigen antibody binding interaction
Sheep anti Human salivary amylase Antibody
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Antibody is raised in
Sheep
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Antibody s reacts with
Human
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Antibody s reacts with these species
The antiSerum does not cross-react with any other Human plasma proteins as tested in gel-diffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
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Antibody s specificity
Precipitating polyclonal Sheep antiSerum to Human salivary amylase.
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Research interest
Enzymes
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Application
Immunoprecipitation
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Antibody s suited for
Immunoprecipitation. In immunoelectrophoresis in agarose-plates use 2 μl human saliva or equivalent against 120 μl antiserum. In double radial immunodiffusion use a rosette arrangement with 10 μl antiserum in 3 mm diameter center well and 2 μl plasma samples (neat and serially diluted) in 2 mm diameter peripheral wells. Precipitin titre not less than 1:32 when tested against normal human saliva in agar-block immunodiffusion titration.
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Storage
The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day. Lyophilized at +4°C--at least 10 years. Reconstituted at or below -20°C--3-5 years. Reconstituted at +4°C--7 days.
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Relevant references
no information yet
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Protein number
P04745
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
