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Alternative name1
Anti-Rabbit Anti-Flag
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Alternative name2
Rabbit Anti-Flag
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Alternative name3
Lapin Anti-Flag
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Alternative name4
Flag
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Alternative name5
N/A
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Other names
FlaG; N/A; N/A; N/A; FlaG
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Gene name
N/A
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Gene name synonims
N/A
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Other gene names
N/A; flaG; N/A; Clst_2467
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Category
Antibodies
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Clonality
Polyclonal
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Immunoglobulin isotype
IgG, kappa
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Clone
2F11-4H10
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Host organism
Rabbit
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Species reactivity
N/A
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Specificity and cross reactivity
N/A
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Purification method
Protein A purified
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Form Appearance
N/A
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Concentration
1 miligram per 1 mililiter
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Storage and shipping
Lyophilized antibodies may be transported at ambient temperature and stored for short terms at +4 degrees Celsius and at -20 for long term storage. Antibodies in liquid form can be shipped and stored for short terms at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol has to added and the vial must be stored at -20°C.
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Tested for
Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF), ELISA (EIA)
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Description
productone is a polyclonal antibody of high purity and binding affinity for the antigen that it is risen against. Properly used, this antibody will ensure excellent and reproducible results with guaranteed success for the applications that it is tested in. Polyclonal antibodies have series of advantages - larger batches can be supplied at a time, they are inexpensive to manufacture and respectively to buy, the time needed for production is considerably shorter. Polyclonal antibodies generally are more stable and retain their reactivity under unfavorable conditions. To obtain more detailed information on productone, please, refer to the full product datasheet.
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Advisory
In order to retain the quality and the affinity of productone unchanged, please, avoid cycles of freezing and thawing. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
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Additional description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv. These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
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Conjugation
Flag