RAT ANTI DOG CD4:FITC Antibody

• Catalog number
  GEN214750
• Price
  Please ask
• Size
  100 Tests

• Also known as
  CD4
• Other names
  T-cell surface glycoprotein CD4; T-cell surface glycoprotein CD4; T-cell surface glycoprotein CD4; CD4 antigen (p55); T-cell surface antigen T4/Leu-3; N/A; T-cell surface antigen T4/Leu-3
• Category
  Antibodies
• Subcategory
  Mnoclonal antibodies
• Gene name
  CD4
• Gene name synonims
  N/A
• Other gene names
  CD4; CD4; N/A; N/A
• Clonality
  Monoclonal
• Immunoglobulin isotype
  IgG2a
• Clone
  YKIX302.9
• Host organism
  Rat (Rattus norvegicus)
• Species reactivity
  Dog; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
• Specificity and cross reactivity
  CD4 This item recognises the canine CD4 cell surface antigen. The antibody has been shown to block MHCII dependent T cell responses in vivo and in vitro. In the dog CD4 is expressed by neutrophils as well as by lymphocytes subsets.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
• Purification method
  N/A
• Form Appearance
  FITC (Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid)
• Concentration
  IgG concentration 0.1 miligram per 1 mililiter
• Storage and shipping
  Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term. the antibody should be stored undiluted. Storage in frost free freezers is not recommended. the antibody is photosensitive and should be protected from light. Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided. If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use. Shelf Life: 18 months from date of dispatch.
• Tested applications
  Flow Cytometry (FACS)
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. CD4+ T helper cell marker or cluster differentiator of white blood cells in FACS or flow cytometry. Used offer coupled to magnetic beads. GENTAUR makes custom magnetic bead couplings on 50 and 250 nm beads for certain clones.
• Properties
  If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Canine or Canis Lupus is mostly Beagle used for drug research. MBS Monoclonals produces ELISA test kits and polyclonal antibodies. This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• About
  Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
• Latin name
  Rattus norvegicus
• French translation
  anticorps

• Gene target
  CD4  
• Gene symbol
  CD4
• Short name
  ANTI CD4:FITC Antibody
• Technique
  Antibody, anti, FITC, antibody to, antibodies against human proteins, antibodies for, Fluorescein
• Host
  Rat
• Label
  FITC
• Species
  Dog, Dogs, Rats
• Alternative name
  Anti-RAT ANTI DOG CD4:FITC
• Alternative technique
  antibodies, fluorescine
• Alternative to gene target
  CD4 molecule, CD4mut, CD4 and IDBG-15034 and ENSG00000010610 and 920, protein homodimerization activity, Cell surfaces, Cd4 and IDBG-188351 and ENSMUSG00000023274 and 12504, CD4 and IDBG-640247 and ENSBTAG00000003255 and 407098

Gene info

• Identity
  HGNC:1678
• Gene
  CD4
• Long gene name
  CD4 molecule
• Synonyms gene name
  • CD4 antigen (p55)
  • T-cell surface glycoprotein CD4
• GenBank acession
  • M35160
• Locus
  12p13.31
• Discovery year
  1986-01-01
• Entrez gene record
  920
• RefSeq identity
  • NM_000616
• Classification
  • CD molecules
  • C2-set domain containing
  • V-set domain containing
• VEGA ID
  OTTHUMG00000168514

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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