Lymphoid Enhancer Factor 1 (LEF1, Lymphoid Enhancer-binding Factor 1, DKFZp586H0919, HGNC:6551, T cell Specific Transcription Factor 1 alpha, TCF1 alpha)
-
Catalog numberGEN600490
-
PricePlease ask
-
Size100ug
-
-
Also known asLymphoid Enhancer Factor 1
-
Other nameslymphoid enhancer-binding factor 1 isoform 1; Lymphoid enhancer-binding factor 1; lymphoid enhancer-binding factor 1; TCF1-alpha; T cell-specific transcription factor 1-alpha; lymphoid enhancer-binding factor 1; T cell-specific transcription factor 1-alpha
-
CategoryAntibodies
-
SubcategoryMnoclonal antibodies
-
Gene nameLEF1; LEF1; LEF-1; TCF10; TCF7L3; TCF1ALPHA; LEF-1; TCF1-alpha
-
Gene name synonimsLEF1; LEF1; LEF-1; TCF10; TCF7L3; TCF1ALPHA; LEF-1; TCF1-alpha
-
Other gene namesLEF1; LEF1; LEF-1; TCF10; TCF7L3; TCF1ALPHA; LEF-1; TCF1-alpha
-
ClonalityMonoclonal
-
Immunoglobulin isotypeIgG1
-
Clone6D538
-
Host organismMouse (Mus musculus)
-
Species reactivityHuman (Homo sapiens), Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
-
Specificity and cross reactivityRecognizes human and mouse LEF. Does not cross-react with T cell factor 3 (TCF3) or T cell factor 4 (TCF4). Detects a band of ~68kDa by Western blot.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
-
Purification methodHighly Purified by size exclusion chromatography.
-
Form AppearanceSupplied as a liquid in PBS, 0.1% sodium azide, PEG and sucrose, before the addiition of glycerol to 40%.
-
ConcentrationN/A
-
Storage and shippingStore the antibody at +4 degrees Celsius for short term storage.. Long-term storage should be done after aliquoting the antibody and freezing the aliqotes at -20 degrees Celsius. Under these conditions the aliquots will be stable for at least one year if kept at -20 °C. Prior to use, briefly centrifuge the original vial after thawing and before lid. If further dilution(s) are needed, they can be done with the appropriate assay buffer.
-
Tested applicationsWestern Blot (WB), Immunocytochemistry (ICC)
-
DescriptionThe Lymphoid Enhancer Factor 1 (LEF1, Lymphoid Enhancer-binding Factor 1, DKFZp586H0919, HGNC:6551, T Specific Transcription Factor 1 alpha, TCF1 alpha) is a α- or alpha protein sometimes glycoprotein present in blood. For cells, cell lines and tissues in culture till half confluency. Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells. The activation of transcription factor subunits is the first step of gene expression, in which a particular segment of DNA is copied into RNA (mRNA) by the enzyme RNA polymerases. Transcription factors, unites and elongations can be RNA and DNA nucleic acids, base pairs of nucleotides . Converting from DNA to RNA is made by enzymatic reactions. During transcription, a DNA sequence is read by an RNA polymerase, which produces a complementary, anti-parallel RNA strand called a primary transcript. Transcriptions are key functions in signal transduction pathways. Signaling ligand binding transcription factors play an important role in transduction cascades.
-
Gene target
-
Gene symbolLEF1-AS1, LEF1, HNF1A
-
Short nameLymphoid Enhancer Factor 1 (LEF1, Lymphoid Enhancer-binding Factor 1, DKFZp586H0919, HGNC:6551, T Specific Transcription Factor 1 alpha, TCF1 alpha)
-
Alternative nameAnti-Lymphoid Enhancer Factor 1 (LEF1, Lymphoid Enhancer-binding Factor 1, DKFZp586H0919, HGNC:6551, T cell Specific Transcription Factor 1 alpha, TCF1 alpha)
-
Alternative to gene targetlymphoid enhancer-binding factor 1, LEF1 and IDBG-33529 and ENSG00000138795 and 51176, DNA binding, nuclei, Lef1 and IDBG-194292 and ENSMUSG00000027985 and 16842, LEF1 and IDBG-640560 and ENSBTAG00000006844 and 535399
-
Tissuecell
-
Gene info
-
Identity
-
Gene
-
Long gene nameLEF1 antisense RNA 1
-
Synonyms gene name
- LEF1 antisense RNA 1 (non-protein coding)
-
Locus
-
Discovery year2012-05-28
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Antisense RNAs
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namelymphoid enhancer binding factor 1
-
Synonyms gene name
- lymphoid enhancer-binding factor 1
-
Synonyms
-
Locus
-
Discovery year1991-06-05
-
Entrez gene record
-
Pubmed identfication
-
Classification
- TCF/LEF transcription factor family
- Wnt enhanceosome complex
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameHNF1 homeobox A
-
Synonyms gene
-
Synonyms gene name
- transcription factor 1, hepatic; LF-B1, hepatic nuclear factor (HNF1), albumin proximal factor
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year1990-02-12
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- HNF class homeoboxes
-
VEGA ID
-
Locus Specific Databases