Anti-Mouse CD27 FITC (Clone LG.3A10) (Armenian Hamster IgG1)

• Catalog number
  GEN520087
• Price
  Please ask
• Size
  100ug

• Also known as
  CD27
• Other names
  CD27 antigen; CD27 antigen; CD27 antigen; CD27L receptor; T cell activation antigen S152; T-cell activation antigen CD27; tumor necrosis factor receptor superfamily, member 7; CD27 molecule; CD27L receptor; T-cell activation antigen CD27; T14; Tumor necrosis factor receptor superfamily member 7
• Category
  Antibodies
• Subcategory
  Mnoclonal antibodies
• Gene name
  CD27
• Gene name synonims
  N/A
• Other gene names
  CD27; CD27; T14; S152; Tp55; TNFRSF7; S152. LPFS2; TNFRSF7
• Clonality
  Monoclonal
• Immunoglobulin isotype
  Armenian hamster IgG1
• Clone
  LG.3810
• Host organism
  Armenian Hamster
• Species reactivity
  Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
• Specificity and cross reactivity
  CD27 (Mouse CD27 Receptor); Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
• Purification method
  N/A
• Form Appearance
  FITC
• Concentration
  0.1 miligram per 1 mililiter
• Storage and shipping
  For all formats, store the antibody at +4 degrees Celsius. DO NOT FREEZE PE conjugates. For long term storage (Purified, Biotin, FITC, No Azide), aliquot and freeze unused portion the antibody should be stored at -20 degrees Celsius. in volumes appropriate for single usage. Avoid freeze/thaw cycles.
• Tested applications
  Immunohistochemistry (IHC), Flow Cytometry (FC/FACS) (stimulating)
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes. Armenian of Syrian hamster monoclonal IgGs are useful antibodies for double staining your IHC samples. Using mabs or monoclonal antibodies has a lot of advantages for Flow cytometry.
• Conjugation
  Anti-FITC Antibody
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus

• Gene target
  CD27   3A10   Armenian   IgG1  
• Gene symbol
  CD27-AS1, CD27
• Short name
  Anti-Mouse CD27 FITC (Clone LG 3A10) (Armenian IgG1)
• Technique
  anti-, Mouse, anti, FITC, antibody to, antibodies, Fluorescein, mouses
• Host
  mouse
• Isotype
  IgG1
• Label
  FITC
• Species
  Hamster, Hamsters, Mouses
• Alternative name
  Anti-Anti-Mouse CD27 FITC (Clone LG.3A10) (Armenian Hamster IgG1)
• Alternative technique
  murine, antibodies, fluorescine
• Alternative to gene target
  CD27 molecule, S152 and S152. LPFS2 and T14 and TNFRSF7 and Tp55, CD27 and IDBG-14014 and ENSG00000139193 and 939, cysteine-type endopeptidase inhibitor activity involved in apoptotic process, Extracellular, Cd27 and IDBG-189750 and ENSMUSG00000030336 and 21940, CD27 and IDBG-640494 and ENSBTAG00000014725 and 512514
• Clone name
  LG

Gene info

• Identity
  HGNC:43896
• Gene
  CD27-AS1
• Long gene name
  CD27 antisense RNA 1
• Synonyms gene name
  • CD27 antisense RNA 1 (non-protein coding)
• GenBank acession
  • AK056500
• Locus
  12p13.31
• Discovery year
  2012-04-27
• Entrez gene record
  678655
• RefSeq identity
  • NR_015382
• Classification
  • Antisense RNAs
• VEGA ID
  OTTHUMG00000168315

Gene info

• Identity
  HGNC:11922
• Gene
  CD27
• Long gene name
  CD27 molecule
• Synonyms gene
  • TNFRSF7
• Synonyms gene name
  • tumor necrosis factor receptor superfamily, member 7
• Synonyms
  • S152
  • Tp55
• GenBank acession
  • M63928
• Locus
  12p13.31
• Discovery year
  1992-06-10
• Entrez gene record
  939
• Pubmed identfication
  • 2442250
  • 8530100
• Classification
  • Tumor necrosis factor receptor superfamily
  • CD molecules
• VEGA ID
  OTTHUMG00000168316
• Locus Specific Databases
  • LRG_357

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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