Human IgA ELISpotBASIC kit (HRP)

• Catalog number
  3860-2H
• Price
  Please ask
• Size
  for 4 plates

• Categories
  ELISpot Kits
• Description
  Antigen-specific B cells can be enumerated in two alternative ways; by coating with antigen or by using biotinylated antigen in the detection step. For memory B cells, we recommend pre-stimulation with R848 and recombinant human IL-2 (both included).
• Enzyme Conjugate
  HRP
• Format
  without plates
• Kit Detection Type
  Streptavidin-HRP
• Application
  ELISpot
• Reactivities
  human
• Analytes
  IgA
• Contents
  Capture mAb (MT57),Biotinylated detection mAb (MT20),Biotinylated detection mAb (MT57),Streptavidin-HRP,R848,Recombinant human IL-2
• Datasheet
• Product Header
  Basic ELISpot for enumeration of cells secreting IgA
• GeneID
  NA
• Shelf Life
  18 months
• Properties
  HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples. Immunoglobulin A (IgA, also referred to as sIgA) is an antibody that plays a critical role in immune function in the mucous membranes. More IgA is produced in mucosal linings than all other types of antibody combined. Examples are EMA, ACA ELISA test kits and for diagnostic detection the IgA of Anti-Beta-2 Glycoprotein 1; β2-Glycoprotein 1 Antibodies; Beta 2GP1 Ab, Beta-2 Glycoprotein 1 Antibodies IgG, IgM, and IgAs.
• Conjugation
  HRP Conjugated

• Gene target
  ELISpotBASIC   kit  
• Short name
  IgA ELISpotBASIC kit ( )
• Technique
  IgA, IgAs
• Isotype
  IgA, IgA
• Label
  HRP
• Species
  Human, Humans
• Alternative name
  H. sapiens Immunoglobulin A ELISpotBASIC reagent (horseradish peroxidase)
• Alternative technique
  immunoglobulin-a, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

MeSH Data

• Name
  Enzyme-Linked Immunospot Assay
• Concept
  Scope note: A method of detection of the number of cells in a sample secreting a specific molecule. With this method, a population of cells are plated over top of the immunosorbent substrate that captures the secreted molecules.
• Tree numbers
  • E01.370.225.500.508
  • E05.200.500.508
  • E05.242.551
  • E05.478.566.350.170.500
  • E05.478.566.380.360.500
  • E05.478.583.400.170.500
  • E05.601.470.350.170.500
  • E05.601.470.380.360.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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