Human IgA ELISpotBASIC kit (ALP)
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Catalog number3860-2A
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Price:
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Sizefor 4 plates
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CategoriesELISpot Kits
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DescriptionAntigen-specific B cells can be enumerated in two alternative ways; by coating with antigen or by using biotinylated antigen in the detection step. For memory B cells, we recommend pre-stimulation with R848 and recombinant human IL-2 (both included).
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Enzyme ConjugateALP
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Formatwithout plates
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Kit Detection TypeStreptavidin-ALP
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ApplicationELISpot
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Reactivitieshuman
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AnalytesIgA
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ContentsCapture mAb (MT57),Biotinylated detection mAb (MT20),Biotinylated detection mAb (MT57),Streptavidin-ALP,R848,Recombinant human IL-2
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Product HeaderBasic ELISpot for enumeration of IgA secreting B cells
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GeneIDNA
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Shelf Life18 months
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples. Immunoglobulin A (IgA, also referred to as sIgA) is an antibody that plays a critical role in immune function in the mucous membranes. More IgA is produced in mucosal linings than all other types of antibody combined. Examples are EMA, ACA ELISA test kits and for diagnostic detection the IgA of Anti-Beta-2 Glycoprotein 1; β2-Glycoprotein 1 Antibodies; Beta 2GP1 Ab, Beta-2 Glycoprotein 1 Antibodies IgG, IgM, and IgAs.
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Gene target
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Gene symbolPDLIM3, ASRGL1, ATRNL1, SLPI, CCL27
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Short nameIgA ELISpotBASIC kit (ALP)
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TechniqueIgA, IgAs
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IsotypeIgA, IgA
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SpeciesHuman, Humans
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Alternative nameH. sapiens Immunoglobulin A ELISpotBASIC reagent (ALP)
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Alternative techniqueimmunoglobulin-a, kits
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Alternative to gene targetv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
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Identity
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Gene
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Long gene namePDZ and LIM domain 3
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Synonyms
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GenBank acession
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Locus
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Discovery year2004-02-06
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- PDZ domain containing
- LIM domain containing
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameasparaginase and isoaspartyl peptidase 1
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Synonyms gene name
- asparaginase like 1
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Synonyms
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Synonyms name
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Locus
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Discovery year2001-08-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameattractin like 1
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Synonyms gene name
- attractin-like 1
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Synonyms
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GenBank acession
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Locus
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Discovery year2004-03-04
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- C-type lectin domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesecretory leukocyte peptidase inhibitor
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Synonyms gene name
- secretory leukocyte protease inhibitor (antileukoproteinase)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1998-04-24
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Entrez gene record
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Pubmed identfication
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Classification
- WAP four-disulfide core domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameC-C motif chemokine ligand 27
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Synonyms gene
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Synonyms gene name
- small inducible cytokine subfamily A (Cys-Cys), member 27
- chemokine (C-C motif) ligand 27
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-07-30
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Chemokine ligands
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VEGA ID
MeSH Data
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Name
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ConceptScope note: A method of detection of the number of cells in a sample secreting a specific molecule. With this method, a population of cells are plated over top of the immunosorbent substrate that captures the secreted molecules.
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Tree numbers
- E01.370.225.500.508
- E05.200.500.508
- E05.242.551
- E05.478.566.350.170.500
- E05.478.566.380.360.500
- E05.478.583.400.170.500
- E05.601.470.350.170.500
- E05.601.470.380.360.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data