pECMV-SLC12A3-6×His-FLAG

Catalog number

PVT20558
Price

Please ask
Size

Gentaur supplies 2ug of lyophilized ECMV-SLC12A3-6×His-FLAG Plasmid in powder form at 4°C to be centrifuged at 5000rpm/min for 1min. Add 20μl ddH2O in to the vial of the pECMV-SLC12A3-6×His-FLAG plasmid.

Vectro Map

The pECMV promotor is combined with a SLC12A3 vector sequence and a 6×His gene in the plasmid vector map.
Properties

An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv. These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
Conjugation

Flag, histidine

Identity

HGNC:10912
Gene

SLC12A3
Long gene name

solute carrier family 12 member 3
Synonyms gene name
- solute carrier family 12 (sodium/chloride transporter), member 3
Synonyms
- NCCT
Locus

16q13
Discovery year

1995-10-02
Pubmed identfication
- 8812482
Classification
- MicroRNA protein coding host genes
- Solute carriers
VEGA ID

OTTHUMG00000133284