Goat, L), FITC conjugated

• Catalog number
  AS16 3280
• Price
  Please ask
• Size
  2.0 mg

• Available ordering format
  Liquid
• Immunogen
  Purified Donkey IgG, whole molecule.
• Raised in
  Goat
• Clonality
  Polyclonal
• Clone
  Polyclonal
• Purification
  Affinity purified
• How to reconstitute
  See included datasheet or contact our support service
• Storage condition
  Store at 2-8 °C.
• Verified applications
  Flow cytometry (FACS), Immunomicroscopy (IM)
• Connected products
  other anti-donkey secondary antibodies
• Recommended dilutions for use
  1:20 - 1:2 000 (FACS and IM)
• Molecular weight expected аpparent
  see datasheet
• Verified reactivity
  Donkey IgG
• Possible reactivity
  to be determined
• No reactivity
  to be determined
• Supplementary information
  Contact Gentaur support center at [email protected]
• References
  To be added when available, antibody released in April 2016.
• Scientific context
  Goat anti-Dognkey IgG (H&L) is a secondary antibody, conjugated to FITC, which binds to Donkey IgG (H&L) in immunological assays.
• Notes
  Antibody purity is > 95% based on SDS-PAGE.Affinity purified using solid phase Donkey IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: - heavy (gamma) chains on donkey IgG - light chains on all donkey immunoglobulins . Based on IEP, no reactivity is observed to: - non-immunoglobulin donkey serum immunoglobulins .
• Protein number
  Refer to NCBI
• TAIR number
  Refer to NCBI
• Hyperlink
• Properties
  This agisera Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. agisera FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• Latin name
  Capra aegagrus hircus

• Gene target
  conjugated  
• Short name
  Goat, L), FITC conjugated
• Technique
  FITC, Goat, antibody Conjugates, Fluorescein, goats
• Host
  Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
• Label
  FITC
• Species
  Goats
• Alternative name
  caprine, L), fluorecein coupled
• Alternative technique
  caprine, fluorescine

MeSH Data

• Name
  Cytophotometry
• Concept
  Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
• Tree numbers
  • E01.370.225.500.386
  • E05.196.712.516.600.240
  • E05.200.500.386
  • E05.242.386
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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