OxiSelect™ Intracellular ROS Assay test kit (Green Fluorescence), Trial Size

• Catalog number
  STA-342-T
• Price
  Please ask
• Size
  20 assays

• We send this reagant on
  Room temperature
• The temperatureerature for storage is
  Upon receipt, store the DCFH-DA, DCF and Hydrogen Peroxide at -20ºC. Avoid multiple freeze/thaw cycles. Store the Cell Lysis Buffer at 4ºC.
• Technical file
  more kit.pdf
• SDS or safty data sheet
  details (STA-342-T) GHS.pdf
• Type of reagent
  Oxidative Stress/Damage
• Related techniques
  Oxidative Stress/ Damage, Reactive Oxygen Species (ROS) Assays, Intracellular ROS Assay
• Use of the reagent
  The OxiSelect™ ROS Assay test kit is a cell-based assay for measuring hydroxyl, peroxyl, and other reactive oxygen species activity within a cell. The assay employs the cell-permeable fluorogenic probe DCFH-DA, which diffuses into cells and is deacetylcated by cellular esterases into the non-fluorescent DCFH (Figure 1). In the presence of ROS, DCFH is rapidly oxidized to highly fluorescent DCF. Fluorescence is read on a standard Fluorimetry plate reader.
• Cellular use
  Quick ~1 hour protocol
• Protocol information
  Highly sensitive to 10 pM
• Reagent properties
  Detects the presence of various ROS species
• Living modified organisme in this reagent or LMO
  Not applicable
• Species compatible to
  Humans
• Use of this test kit or product
  Genetics lab
• Antigen
  a specific protein sequence
• Buffer
  The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,90
• Molecular weight
  in kilo Danlto or kDa
• This reagent is purified
  0.97
• Test
  Cellular biology labs supplies other types of Assays as 1. Fluorimetric analysis of fluorescent emission of light by is a form of luminescence. The emitted light has a longer wavelength, and therefore lower energy, than the absorbed radiation for excitation. Fluorescent controls and calibrators can be supplied to mix with the cells. A fluorimeter is needed to do the analysis of the green, red, blue, yellow, orange, deep red fluorescent dyes.

• Gene target
  OxiSelect™   Intracellular   ROS   test   kit   Trial   Size  
• Gene symbol
  ROS1, GUCY2F, GUCY2D
• Short name
  OxiSelect™ Intracellular ROS Assay test kit ( ), Trial Size
• Technique
  intracellular, Assay, assays
• Host
  Assay
• Label
  green
• Alternative name
  OxiSelect™ Intracellular ROS test test reagent (Green Fluorescence), Trial Size
• Alternative technique
  arrays, tests, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Gene info

• Identity
  HGNC:10261
• Gene
  ROS1
• Long gene name
  ROS proto-oncogene 1, receptor tyrosine kinase
• Synonyms gene name
  • v-ros avian UR2 sarcoma virus oncogene homolog 1
  • v-ros UR2 sarcoma virus oncogene homolog 1 (avian)
  • c-ros oncogene 1 , receptor tyrosine kinase
• Synonyms
  • MCF3
  • ROS
  • c-ros-1
• GenBank acession
  • M13599
• Locus
  6q22.1
• Discovery year
  1986-01-01
• Entrez gene record
  6098
• Pubmed identfication
  • 1611909
• Classification
  • Receptor tyrosine kinases
  • Fibronectin type III domain containing
• VEGA ID
  OTTHUMG00000016188
• Locus Specific Databases
  • LRG_997

Gene info

• Identity
  HGNC:4691
• Gene
  GUCY2F
• Long gene name
  guanylate cyclase 2F, retinal
• Synonyms
  • GUC2DL
  • GC-F
  • RetGC-2
  • ROS-GC2
  • CYGF
• Synonyms name
  • guanylate cyclase 2D-like, membrane (retina-specific)
• GenBank acession
  • L37378
• Locus
  Xq22.3-q23
• Discovery year
  1997-12-05
• Entrez gene record
  2986
• Pubmed identfication
  • 8838319
  • 7777544
• RefSeq identity
  • NM_001522
• Classification
  • Guanylate cyclase receptors
• VEGA ID
  OTTHUMG00000022184
• Locus Specific Databases
  • Global Variome shared LOVD

Gene info

• Identity
  HGNC:4689
• Gene
  GUCY2D
• Long gene name
  guanylate cyclase 2D, retinal
• Synonyms gene
  • CORD6
  • LCA
  • GUC2D
  • GUC1A4
• Synonyms gene name
  • cone rod dystrophy 6
  • guanylate cyclase 2D, membrane (retina-specific)
• Synonyms
  • retGC
  • RETGC-1
  • ROS-GC1
  • CYGD
  • LCA1
• Synonyms name
  • rod outer segment membrane guanylate cyclase
  • retinal guanylate cyclase 1
• GenBank acession
  • L26921
• Locus
  17p13.1
• Discovery year
  1993-11-09
• Entrez gene record
  3000
• Pubmed identfication
  • 1356371
  • 12552567
• RefSeq identity
  • NM_000180
• Classification
  • Guanylate cyclase receptors
• VEGA ID
  OTTHUMG00000108169
• Locus Specific Databases
  • Mutations of the Retinal Guanylate Cyclase Gene and the Retinal Guanylate Cyclase-activating Protein Gene

MeSH Data

• Name
  Radioallergosorbent Test
• Concept
  Scope note: An in vitro allergen radioimmunoassay in which allergens are coupled to an immunosorbent. The coupled allergens bind the IgE in the sera of patients which in turn binds radioisotope-labeled anti-IMMUNOGLOBULIN E antibodies.
• Tree numbers
  • E01.370.225.812.735.830
  • E05.200.812.735.830
  • E05.478.566.380.810
  • E05.478.566.639.810
  • E05.478.594.760.830
  • E05.601.470.380.810
  • E05.601.470.639.810
• Qualifiers
  ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data

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