Rat CD8 alpha antibody (FITC conjugated)
research antibodies, ELISAs, recombinant proteins, vectors and reagents for laboratory use
The CD8 alpha antibody (FITC conjugated) is a α- or alpha protein sometimes glycoprotein present in blood. Cytotoxic T cells cluster differentiators for flow cytometry. This transmembrane glycoprotein is detected by monoclonals and has a lot of clones available with different affinity.
If you buy Antibodies supplied by genways they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This genways Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. genways FITC is highly sensitive to pH extremes.
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
1. Gene info
Long gene name
Synonyms gene name
- CD8 antigen, alpha polypeptide (p32)
- T-cell surface glycoprotein CD8 alpha chain
Entrez gene record
- CD molecules
- V-set domain containing
Locus Specific Databases
Scope note:Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
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