CD8 Antibody, FITC Conjugated

• Catalog number
  bs-4790R-FITC
• Price
  Please ask
• Size
  0.1ml

• Long name
  CD8 Polyclonal Antibody, FITC Conjugated
• Category
  Conjugated Primary Antibodies
• Conjugation
  FITC
• Host Organism
  Rabbit (Oryctolagus cuniculus)
• Target Antigen
  CD8
• Specificity
  This is a highly specific antibody against CD8.
• Modification
  Unmodified
• Modification site
  None
• Clonality
  Polyclonal
• Clone
  Polyclonal antibody
• Concentration
  1ug per 1ul
• Immunogen_range
  60-110/235
• Subcellular location
  Extracellular, Secreted
• Source
  KLH conjugated synthetic peptide derived from human CD8
• Gene ID number
  925
• Swiss Prot
  P01732
• Tested applications
  FCM, IF(IHC-P)
• Recommended dilutions
  FCM(1:20-100), IF(IHC-P)(1:50-200)
• Crossreactivity
  Human
• Crossreactive species details
  Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
• Antigen background
  Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
• Purification method
  This antibody was purified via Protein A.
• Storage conditions
  Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
• Excitation Emission
  494nm/518nm
• Synonyms
  CD8; MAL; p32; Leu2; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2; CD8a
• Properties
  If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.
• Description
  Cytotoxic T cells cluster differentiators for flow cytometry. This transmembrane glycoprotein is detected by monoclonals and has a lot of clones available with different affinity.
• Additional conjugation
  Anti-FITC Antibody
• French translation
  anticorps

• Gene target
  CD8   Conjugated  
• Gene symbol
  CD8A
• Short name
  Anti-CD8
• Technique
  Antibody, FITC, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein
• Isotype
  Immunoglobulin G (IgG)
• Label
  FITC
• Alternative name
  Anti-CD8 PAb FITC
• Alternative technique
  antibodies, fluorescine

Gene info

• Identity
  HGNC:1706
• Gene
  CD8A
• Long gene name
  CD8a molecule
• Synonyms gene
  • CD8
• Synonyms gene name
  • CD8 antigen, alpha polypeptide (p32)
  • T-cell surface glycoprotein CD8 alpha chain
• Locus
  2p11.2
• Discovery year
  1986-01-01
• Entrez gene record
  925
• Pubmed identfication
  • 1541829
• RefSeq identity
  • NM_001768
• Classification
  • V-set domain containing
  • CD molecules
• VEGA ID
  OTTHUMG00000130265
• Locus Specific Databases
  • CD8Abase: Mutation registry for CD8 deficiency
  • LRG_44

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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