eNOS (Thr495) Antibody, Cy3 Conjugated
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Catalog numberbs-3731R-Cy3
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Price:
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Size0.1ml
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Long nameeNOS (Thr495) Polyclonal Antibody, Cy3 Conjugated
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CategoryConjugated Primary Antibodies
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ConjugationCy3
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Host OrganismRabbit (Oryctolagus cuniculus)
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Target AntigeneNOS Thr495
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SpecificityThis is a highly specific antibody against eNOS Thr495.
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ModificationPhosphorylation
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Modification siteThr495
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ClonalityPolyclonal
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ClonePolyclonal antibody
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Concentration1ug per 1ul
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Immunogen_range470-520/1203
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Subcellular locationCytoplasm, Cell membrane
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SourceKLH conjugated synthetic phosphopeptide derived from human eNOS around the phosphorylation site of Thr495
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Gene ID number4846
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Swiss ProtP29474
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Tested applicationsIF(IHC-P)
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Recommended dilutionsIF(IHC-P)(1:50-200)
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CrossreactivityHuman, Mouse, Rat
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Crossreactive species detailsDue to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Antigen backgroundProduces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.
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Purification methodThis antibody was purified via Protein A.
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Storage conditionsKeep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
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Excitation Emission512,550nm/570,615nm
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SynonymseNOS; ECNOS; Nitric oxide synthase, endothelial; Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; NOS type III; NOSIII; NOS3
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PropertiesIf you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Cy3 antibodies are excited by the 488-nanometer wave of an argon laser and the 633-nanometer line of a helium-neon diode laser. 1 of the Anti-eNOS Thr495 can be used in flow cytometry but typically shows lower fluorescence intensity comparable to that of PE or APC. This Bioss Primary Conjugated Antibodies antibody is well suited for fluorescent microscopy.
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Additional conjugationcy3 conjugation kit
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French translationanticorps
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Gene target
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Short nameAnti-eNOS Thr495
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TechniqueAntibody, antibodies against human proteins, antibodies for, antibody Conjugates
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IsotypeImmunoglobulin G (IgG)
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LabelCy3
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Alternative nameAnti-eNOS Thr495 PAb Cy3
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Alternative techniqueantibodies
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data