eNOS (Thr113) Antibody, Cy3 Conjugated

  • Catalog number
    bs-3589R-Cy3
  • Price
    Please ask
  • Size
    0.1ml
  • Long name
    eNOS (Thr113) Polyclonal Antibody, Cy3 Conjugated
  • Category
    Conjugated Primary Antibodies
  • Conjugation
    Cy3
  • Host Organism
    Rabbit (Oryctolagus cuniculus)
  • Target Antigen
    eNOS Thr113
  • Specificity
    This is a highly specific antibody against eNOS Thr113. This phosphorylation site is homologous across the listed species; this phosphorylation site does not exist in human due to a Thr-Ser substitution.
  • Modification
    Phosphorylation
  • Modification site
    Thr113
  • Clonality
    Polyclonal
  • Clone
    Polyclonal antibody
  • Concentration
    1ug per 1ul
  • Immunogen_range
    80-130/1202
  • Subcellular location
    Cytoplasm, Cell membrane
  • Source
    KLH conjugated synthetic phosphopeptide derived from mouse eNOS around the phosphorylation site of Ser113
  • Gene ID number
    18127
  • Swiss Prot
    P70313
  • Tested applications
    IF(IHC-P)
  • Recommended dilutions
    IF(IHC-P)(1:50-200)
  • Crossreactivity
    Mouse, Rat
  • Crossreactive species details
    Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
  • Antigen background
    Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. May play a significant role in normal and abnormal limb development.
  • Purification method
    This antibody was purified via Protein A.
  • Storage conditions
    Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
  • Excitation Emission
    512,550nm/570,615nm
  • Synonyms
    eNOS; Nos-3; ecNOS; 2310065A03Rik; Nitric oxide synthase, endothelial; Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; NOS type III; NOSIII; Nos3
  • Properties
    If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Cy3 antibodies are excited by the 488-nanometer wave of an argon laser and the 633-nanometer line of a helium-neon diode laser. 1 of the Anti-eNOS Thr113 can be used in flow cytometry but typically shows lower fluorescence intensity comparable to that of PE or APC. This Bioss Primary Conjugated Antibodies antibody is well suited for fluorescent microscopy.
  • Additional conjugation
    cy3 conjugation kit
  • French translation
    anticorps
  • Gene target
  • Short name
    Anti-eNOS Thr113
  • Technique
    Antibody, antibodies against human proteins, antibodies for, antibody Conjugates
  • Isotype
    Immunoglobulin G (IgG)
  • Label
    Cy3
  • Alternative name
    Anti-eNOS Thr113 PAb Cy3
  • Alternative technique
    antibodies
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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