HEPES Buffer 1 M - 100ml
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Catalog numberLM-S2030/100
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Price:
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Size15X100ml
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DescriptionHEPES Buffer 1 M - 100ml is a buffer solution that is used in cell culture media.
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Shipping conditions-20°C or dry ice
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Storage+2/+8°C
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Shelflife36 months
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StateLiquid
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Chemical descriptionBuffer solution
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AppearancePhysical state at 20 ºC: Liquid ; Appearance: Fluid ; Colour: Colourless
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Density at 20 ºC1107 kg/m³
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Relative density at 20 ºC1.107
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PH7.3 ± 0.3
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Osmolality> 1000 mOsm/kg
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Endotoxin< 1 EU/ml
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Composition238.31 g/l of HEPES in distilled water
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SterilitySterile
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Personal precautionsWear clothes adapted to the manipulation of serum to avoid contamination (e.g. : gloves, mask, hygiene cap, overall…)
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UsesUsage biotechnologique. Uniquement pour usage professionnel.
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OrderingTo order HEPES Buffer 1 M - 100ml, please use the Cat. Nr.LM-S2030/100 and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
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TipsOur specialists recommend you to follow carefully the pre-registered instructions for HEPES Buffer 1 M - 100ml
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Additional descriptionBuffering solutions are useful to keep the pH range sable when using this reagent of Biosera.
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Buffer25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.
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Gene target
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Short name1 M - 100ml
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Alternative nameHEPES solution 1 M - 100ml
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