Rat Anti Mouse CD49d Antibody-FITC
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Catalog numberMO49DF(V100)
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PricePlease ask
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Size0,1 mg
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DescriptionRat Anti Mouse CD49d Antibody-FITC is a mouse monoclonal antibody, specific for [R1-2] clones, this reagent was conjugated with FITC format and reported for use in flow cytometric analysis to react with CD49d glycoproteins, CD49d found in all T, B, dendritic, mast, and Nk cells, also in expressed in thymocyte/monocytes, and eosinophils, basophils, CD49d plays a role of adhesion and costimulation of T cells.
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CloneR1-2
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ConjugateFITC
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Gene NameCD49d
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Other Names of geneα4 integrin, VLA-4 α chain, integrin α4, ITGA4
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Concentration500 µg/mL
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ApplicationFlow Cytometry Method
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ImmunogenAKR/Cum mouse spontaneous T lymph oma line TK1
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FormulationAqueous buffered solution containing protein stabilizer, and ≤0.09% sodium azide (NaN3).
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Storage2-8°C, store in dark, do not freeze
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ExpirationAfter 1 year
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RemarkFor Recherche Use Only.
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Additional descriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. This is against a protein of mus musculus directed antibody.
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PropertiesIf you buy Antibodies supplied by Immunostep they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Immunostep Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Immunostep FITC is highly sensitive to pH extremes.
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ConjugationAnti-FITC Antibody
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus, Rattus norvegicus
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AboutRats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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French translationanticorps
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Gene target
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Gene symbolITGA4
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Short nameAnti Mouse CD49d Antibody-FITC
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Techniqueanti mouse, Antibody, Mouse, anti, FITC, antibody to, anti mouse antibodies, antibodies against human proteins, antibodies for, Fluorescein, mouses
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HostRat
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IsotypeRat IgG2b
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LabelFITC
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SpeciesMouse, Mouses, Rats
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Alternative nameRat CD49d Mouse Monoclonal Antibody [R1-2]-FITC
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Alternative techniqueantibodies, murine, fluorescine
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Gene info
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Identity
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Gene
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Long gene nameintegrin subunit alpha 4
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Synonyms gene
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Synonyms gene name
- integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor)
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Synonyms
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Synonyms name
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Locus
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Discovery year1991-08-06
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Entrez gene record
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Pubmed identfication
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Classification
- CD molecules
- Integrin alpha subunits
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The number of CD4-POSITIVE T-LYMPHOCYTES per unit volume of BLOOD. Determination requires the use of a fluorescence-activated flow cytometer.
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Tree numbers
- E01.370.225.500.195.107.595.500.150
- E01.370.225.625.107.595.500.150
- E05.200.500.195.107.595.500.150
- E05.200.625.107.595.500.150
- E05.242.195.107.595.500.150
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data