Rat Anti Mouse CD49d Antibody-Biotin
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Catalog number
MO49DB(V100)
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Price
Please ask
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Size
0,1 mg
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Description
Rat Anti Mouse CD49d Antibody-Biotin is a mouse monoclonal antibody, specific for [R1-2] clones, this reagent was conjugated with Biotin format and reported for use in flow cytometric analysis to react with CD49d glycoproteins, CD49d found in all T, B, dendritic, mast, and Nk cells, also in expressed in thymocyte/monocytes, and eosinophils, basophils, CD49d plays a role of adhesion and costimulation of T cells.
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Clone
R1-2
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Conjugate
Biotin
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Gene Name
CD49d
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Other Names of gene
α4 integrin, VLA-4 α chain, integrin α4, ITGA4
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Concentration
500 µg/mL
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Application
Flow Cytometry Method
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Immunogen
AKR/Cum mouse spontaneous T lymph oma line TK1
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Formulation
Aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium azide (NaN3).
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Storage
2-8°C, store in dark, do not freeze
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Expiration
After 1 year
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Remark
For Recherche Use Only.
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Additional description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. This is against a protein of mus musculus directed antibody.
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Properties
If you buy Antibodies supplied by Immunostep they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Conjugation
Biotinylated
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus, Rattus norvegicus
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About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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French translation
anticorps
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Gene target
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Gene symbol
ITGA4
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Short name
Anti Mouse CD49d Antibody-
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Technique
anti mouse, Antibody, Mouse, anti, antibody to, anti mouse antibodies, antibodies against human proteins, antibodies for, mouses
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Host
Rat
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Isotype
Rat IgG2b
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Label
Biotin
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Species
Mouse, Mouses, Rats
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Alternative name
Rat CD49d Mouse Monoclonal Antibody [R1-2]-Biotin
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Alternative technique
antibodies, murine
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Gene info
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Identity
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Gene
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Long gene name
integrin subunit alpha 4
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Synonyms gene
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Synonyms gene name
- integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor)
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Synonyms
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Synonyms name
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Locus
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Discovery year
1991-08-06
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Entrez gene record
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Pubmed identfication
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Classification
- CD molecules
- Integrin alpha subunits
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VEGA ID
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