Mouse-CD103/ CD22/ CD20 Human Antibody [BerACT8/ HIB22/ LT20]-FITC/ PE/ PerCP
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Catalog number
BD-103F22PE20PP2-50T
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Price
Please ask
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Size
50 TEST
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Description
Mouse-CD103/ CD22/ CD20 Human Antibody [BerACT8/ HIB22/ LT20]-FITC/ PE/ PerCP is a human antibody resulting from the mixture of three monclonal antibodies, this reagent was [BerACT8] FITC conjugated, [HIB22] PE conjugated and [LT20] PerCP conjugated, the product is reported for use in flow cytometric analysis for three color, this antibody reacts with antigen of CD103, CD22, and CD20 to identifymucosa lymphocyte, and B lymphocytes.
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Conjugated
FITC/ PE/ PerCP
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Clone
BerACT8/ HIB22/ LT20
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Application
Flow Cytomtry Method
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Genes name
CD103/ CD22/ CD20
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Formulatin
Phosphate buffred solution, 0,09% sodium Azide, may contain carrier protein-stabilizer
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Expiration
After 1 year
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Storage
2-8°C, store in dark, do not freeze
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Remark
For Recherche Use Only.
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Properties
If you buy Antibodies supplied by Immunostep they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Immunostep Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Immunostep FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Conjugation
Anti-FITC Antibody
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Conjugate
Peridinin chlorophyll (PerCP)
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French translation
anticorps
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Gene target
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Gene symbol
ITGAE, CD22, MS4A1
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Short name
Mouse-CD103/ CD22/ CD20 Antibody [BerACT8/ HIB22/ LT20]-FITC/ PE/ PerCP
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Technique
Antibody, Mouse, Percp, FITC, antibodies against human proteins, antibodies for, Fluorescein, mouses, Peridinin chlorophyll protein (PerCP) has a 677 nanometer maximum emission, red, when excited at 488 nanometers and the Mouse-CD103/ CD22/ CD20 Antibody [BerACT8/ HIB22/ LT20]-FITC/ PE/ PerCP is detected on the FL3 detector of a FACSCalibur or FACScan. A PerCP dye is also available (PerCP-Cy5.5, also written Cy5.5-PerCP). PerCP is not suited for the high-powered lasing (>150mW) applications, such as on a MoFlo, due to it photobleaching (features/ qualities/ traits). PerCP conjugates can only be received/be gotten from the Becton Dickinson/Pharmingen group of companies or from Immunostep.
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Host
mouse
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Isotype
IgG1, IgG2b, IgG1
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Label
FITC
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Species
Human, Humans, Mouses
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Alternative name
Anti Human CD103/ CD22/ CD20 Antibody-FITC/PE/PerCP
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Alternative technique
antibodies, antibodies-percp, murine, fluorescine
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Alternative to gene target
CD22 molecule, SIGLEC-2 and SIGLEC2, CD22 and IDBG-44001 and ENSG00000012124 and 933, protein binding, Cell surfaces, Cd22 and IDBG-171574 and ENSMUSG00000030577 and 12483, CD22 and IDBG-635610 and ENSBTAG00000011887 and 514582
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Gene info
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Identity
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Gene
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Long gene name
integrin subunit alpha E
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Synonyms gene name
- integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1997-06-09
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- CD molecules
- Integrin alpha subunits
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
CD22 molecule
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Synonyms gene name
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1991-06-06
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- CD molecules
- MicroRNA protein coding host genes
- C2-set domain containing
- Sialic acid binding Ig like lectins
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VEGA ID
Gene info
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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