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Mouse anti CD20 antibody conjugated to FITC

  • Catalog number
    0202
  • Product name
    Mouse anti CD20 antibody conjugated to FITC
  • Size
    100 Tests
  • Category
    Primary Antibodies
  • Long description
    Identification of human B cells associated with approximately 10% of peripheral blood lymphocytes.
  • Antibody come from
    CD20=Derived from the hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with the LB lymphoblastoid cell line.
  • Other description
    Provided as sterile filtered solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein. Protein A/G Chromatography
  • Clone
    DFA-7
  • Isotype
    IgG1
  • Antigen antibody binding interaction
    Mouse anti CD20 antibody conjugated to FITC Antibody
  • Antibody is raised in
    Mouse
  • Antibody s reacts with
    Human
  • Antibody s reacts with these species
    This antibody doesn't cross react with other species
  • Label
    FITC
  • Antibody s specificity
    No Data Available
  • Research interest
    CD Marker
  • Application
    Flow Cytometry
  • Antibody s suited for
    PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2¼ to 8¼C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature (20¼C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturerÕs instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
  • Storage
    4ºC
  • Relevant references
    1. In vivo and in vitro expression of myeloid antigens on B-lineage acute lymphoblastic leukemia cells. Leukemia 1991 Ja;5(1):1925 Hara J; Kawa-Ha K; Yumura-Yagi K; Kurahashi H; Tawa A; Ishihara S; Inoue M; Murayama N; Okada S _x000B__x000B_2. Activation of dense human tonsilar B cells. Induction of c-myc gene expression via two distinct signal transduction pathways. J Immunol 1991 Feb ;146(3):846-53 White MW; McConnell F; Shu GL; Morris DR; Clark EA _x000B__x000B_3. Differential effects of low and high concentrations of interleukin 6 on human B cells. Eur J Immunol 1990 No;20(11):2389-93 Levy Y; Fermand JP; Brouet JC _x000B__x000B_4. Immunophenotypes in 'classical' B-cell chronic lymphocytic leukemia. Correlation with normal cellular counterpart and clinical findings. Cancer 1990 Oct 1;66(8):1738-42 Baldini L; Cro L; Cortelezzi A; Calori R; Nobili L; Maiolo AT; Polli EE _x000B__x000B_5. B-cell differentiation following autologous, conventional, or T-cell depleted bone marrow transplantation: a recapitulation of normal B-cell ontogeny. Blood 1990 Oct 1;76(8):1647-56 Small TN; Keever CA; Weiner-Fedus S; Heller G; O'Reilly RJ; Flomenberg N _x000B__x000B_6. Phenotypic analysis of a large number of normal human bone marrow sample by flow cytometry. Blut 1990 No;61(5):271-7 Andreoni C; Rigal D; Bonnard M; Bernaud J _x000B__x000B_7. Identification and characterization of plasma cells in normal human bone marrow by high-resolution flow cytometry. Blood 1990 Nov ;76(9):1739-47 Terstappen LW; Johnsen S; Segers-Nolten IM; Loken MR
  • Protein number
    see ncbi
  • Warnings
    This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
  • Antibody s link
    Antibody_s_link
  • Gene target
    CD20 conjugated
    • Gene info
    Locus Specific Databases:LRG_140
  • Gene symbol
    MS4A1
  • Host
    mouse
  • isotype filter
    • IgG1
  • Short name
    Mouse anti CD20 antibody conjugated FITC
  • label filter
    • FITC
  • technique filter
    • Antibody
    • Mouse
    • anti
    • FITC
  • Technique
    Antibody, Mouse, anti, FITC, antibody to, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein, mouses
  • Alternative technique
    antibodies, murine, fluorescine