FITC-Conjugated Rat IgG1 Isotype Control
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Catalog numberRICIGG1-02MG
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Price:
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Size200 µg
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CloneRTK2071
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ConjugatedFITC
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Tested applicationFlow Cytometry
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Long descriptionThis FITC-Conjugated Rat IgG1 Isotype Control was affinity purified from rabbit serum. It does not cross-react with any human or animal antigen
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Storage bufferPBS, 0,09% sodium Azide, may contain carrier protein-stabilizer
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Shipping conditionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Technical datasheetPlease contact us to receive the latest datasheet, the certificate of analysis or the MSDS
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Regulation statusThis antibody is intended for Research Use Only.
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DescriptionIsotype or positive controls by peptides, antibodies and deactivated samples.
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PropertiesThis Immunostep Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Immunostep FITC is highly sensitive to pH extremes. Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.
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ConjugationAnti-FITC Antibody
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Groupisotypes
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AboutRats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin nameRattus norvegicus
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Gene target
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Short nameFITC-Conjugated IgG1 Isotype Control
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TechniqueControl, Isotype, FITC, antibody Conjugates, controls, Fluorescein, Isotypes and controls
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HostRat
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IsotypeIgG1, IgG1
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LabelFITC
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SpeciesRat, Rats
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Alternative namefluorecein-coupled Rat IgG1 isoform reference
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Alternative techniquecontrol, controls, fluorescine
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Tissuecontrol
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MeSH Data
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Name
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ConceptScope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data