Anti Human CD45RA/CD45RO/CD3/CD8 Antibody-FITC, PE, PerCP, APC
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Catalog number
45RAF245ROPE3PP18A3-50T
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Price
Please ask
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Size
50 TEST
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Description
Anti Human CD45RA/CD45RO/CD3/CD8 Antibody-FITC, PE, PerCP, APC is a human antibody resulting from the combination of four monoclonal antibodies, this reagent was [HI100] FITC conjugated, [UCHL1] PE conjugated, [33-2A3] PerCP conjugated and [MEM-31] APC conjugated, the product is reported for use in flow cytometric analysis for four color, this antibody react with CD45, CD3, and CD8 antigen to identify mature T lymphocytes, and leukocytes and some thymocytes.
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Conjugated
FITC, PE, PerCP, APC
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Clone
HI100, UCHL1, 33-2A3, MEM-31
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Application
Flow Cytomtry Method
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Genes name
CD45RA/CD45RO/CD3/CD8
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Formulatin
Phosphate buffred solution, 0,09% sodium Azide, may contain carrier protein-stabilizer
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Expiration
After 1 year
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Storage
2-8°C, store in dark, do not freeze
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Remark
For Recherche Use Only.
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Additional description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk. Cytotoxic T cells cluster differentiators for flow cytometry. This transmembrane glycoprotein is detected by monoclonals and has a lot of clones available with different affinity.
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Properties
If you buy Antibodies supplied by Immunostep they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Immunostep Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Immunostep FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Conjugation
Anti-FITC Antibody
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Conjugate
Peridinin chlorophyll (PerCP)
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French translation
anticorps
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Gene target
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Gene symbol
CD8A, APC
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Short name
Anti CD45RA/CD45RO/CD3/CD8 Antibody-FITC, PE, PerCP, APC
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Technique
anti human, Antibody, Percp, anti, FITC, antibody to, anti human antibodies, antibodies against human proteins, antibodies for, Fluorescein, Peridinin chlorophyll protein (PerCP) has a 677 nanometer maximum emission, red, when excited at 488 nanometers and the Anti CD45RA/CD45RO/CD3/CD8 Antibody-FITC, PE, PerCP, APC is detected on the FL3 detector of a FACSCalibur or FACScan. A PerCP dye is also available (PerCP-Cy5.5, also written Cy5.5-PerCP). PerCP is not suited for the high-powered lasing (>150mW) applications, such as on a MoFlo, due to it photobleaching (features/ qualities/ traits). PerCP conjugates can only be received/be gotten from the Becton Dickinson/Pharmingen group of companies or from Immunostep.
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Isotype
IgG1, IgG2, IgG2a, IgG2a
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Label
FITC
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Species
Human, Humans
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Alternative name
Mouse-CD45RA/CD45RO/CD3/CD8 Human Antibody [HI100, UCHL1, 33-2A3, MEM-31]-FITC, PE, PerCP, APC
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Alternative technique
antibodies, antibodies-percp, fluorescine
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Alternative to gene target
adenomatous polyposis coli, BTPS2 and DP2 and DP2.5 and DP3 and GS and PPP1R46, APC and IDBG-37007 and ENSG00000134982 and 324, microtubule plus-end binding, nuclei, Apc and IDBG-134573 and ENSMUSG00000005871 and 11789, BT.25770 and IDBG-644937 and ENSBTAG00000018852 and 533233
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Gene info
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Identity
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Gene
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Long gene name
CD8a molecule
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Synonyms gene
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Synonyms gene name
- CD8 antigen, alpha polypeptide (p32)
- T-cell surface glycoprotein CD8 alpha chain
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Locus
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Discovery year
1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- V-set domain containing
- CD molecules
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VEGA ID
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Locus Specific Databases
Gene info
MeSH Data
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Name
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Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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