Thioglycollate Medium w/ Hemin and Vitamin K

Description

Thioglycollate Medium w/ Hemin and Vitamin K is employed for routine cultivation of fastidious anaerobic microorganisms. It can also be used for blood culturing and studying fermentation reactions.
Other Product
Alternative Name

NA
Type

Dehydrated Culture Media
Origin

Animal
Classification

Bacterial
Composition g L

Tryptose 15.000 Yeast extract 10.000 Sodium thioglycollate 0.500 Sodium chloride 2.500 L-Cystine hydrochloride 0.500 Sodium bicarbonate 0.400 Resazurin 0.001 Hemin 0.005 Vitamin K 0.0005 Agar 0.750 Final pH ( at 25°C) 7.2±0.2
Quantity required per litre

29,65 g
Appearance

Cream to yellow homogeneous free flowing powder
Colour and Clarity of prepared medium

Amber coloured clear to very slightly opalescent fluid with upper 10% or less medium pink on standing
Cultural response

Cultural characteristics observed after an incubation at 35-37°C for 18-48 hours.
Storage

Store dehydrated medium and the prepared medium at 2 - 8°C
Shelf Life

Use before expiry date on the label.
Technical Data
Data Sheet
Additional description

There are two major types of growth media those used for cell culture , which use specific cell types derived from plants or animals, and microbiological culture, which are used for growing microorganisms, such as bacteria or yeast. The most common growth media for microorganisms are nutrient broths and agar plates with antibiotics; specialized media are sometimes required for microorganism and cell culture growth.
Group

media

Name

Primary Cell Culture
Concept

Scope note: The initial culturing of cells derived directly from fresh TISSUES.
Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data