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Description
RPMI-1640 With L-Glutamine, 2gms per liter Glucose, 0.165 moles per liter MOPS buffer Without Sodium bicarbonate, is a yellow colored, clear solution. RPMI were developed for the culture of human normal and neoplastic cells in vitro. It was specifically designed to support the growth of human lymphoblastoid cells in suspension culture. Now the medium is extensively used for a wide range of anchorage dependant cell lines. It is also known to support growth of cells in the absence of serum.
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Shelf life
12 months.
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Storage
Store at 2-8°C away from bright light
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Appearance
Yellow colored, clear solution.
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PH
5.30 -5.90
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Osmolality in mOsm Kg H2O
390.00 -430.00
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Sterility
No bacterial or fungal growth is observed after 14 days of incubation, as per USP specification.
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Cultural Response
The growth promotion capacity of the medium is assessed qualitatively by analyzing the cells for the morphology and quantitatively by estimating the cell counts and comparing it with a control medium through minimum three subcultures.
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Endotoxin Content
NMT 5EU/ml
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Uses
Laboratory chemicals, Manufacture of substances
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Personal Protection
Use personnel protective equipment. Wear disposable gloves, dust mask and eye protection. Avoid dust formation.
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Toxicological Information
NA
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Ordering
To order RPMI-1640 With L-Glutamine, 2gms per liter Glucose, 0.165 moles per liter MOPS buffer Without Sodium bicarbonate, please use the Cat. Nr.AL200A-40X500ML and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
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Tips
Our specialists recommend you to follow carefully the pre-registered instructions for RPMI-1640 With L-Glutamine, 2gms per liter Glucose, 0.165 moles per liter MOPS buffer Without Sodium bicarbonate
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Additional description
Buffering solutions are useful to keep the pH range sable when using this reagent of Himedia.
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Buffer
MOPS (3-(N-morpholino)propane sulfonic acid) is a buffer introduced by Good et al. in the 1960s. It is a structural analog to MES.[1] Its chemical structure contains a morpholine ring. HEPES is a similar pH buffering compound that contains a piperazine ring. With a pKa of 7.20, MOPS is an excellent buffer for many biological systems at near-neutral ph.
