Nutrient Mixture F-10 Ham With L-Glutamine and 25mM HEPES buffer Without Sodium bicarbonate
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Catalog numberAT083-5X10X1L
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PricePlease ask
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Size5X10X1L
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DescriptionNutrient Mixture F-10 Ham, With L-Glutamine and 25mM HEPES buffer Without Sodium bicarbonate, is an off-white to creamish white, homogenous powder. It was originally developed for single cell plating of near diploid Chinese hamster ovary (CHO) cells and mouse L-cells. It was designed for clonal growth of CHO cells and chick embryo cells under serum free conditions. It is now widely used for culturing a variety of cells which include human diploid cells and white blood cells for chromosomal analysis
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Shelf lifeUse before expiry date given on the product label.
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StorageStore at 2-8°C away from bright light
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AppearanceOff-white to Creamish white, homogenous powder.
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PH without Sodium Bicarbonate5.60 -6.20
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PH with Sodium Bicarbonate6.40 -7.00
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Osmolality without Sodium Bicarbonate320.00 -360.00
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Osmolality with Sodium Bicarbonate340.00 -380.00
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Cultural ResponseThe growth promotion capacity of the medium is assessed qualitatively by analyzing the cells for the morphology and quantitatively by estimating the cell counts and comparing it with a control medium through minimum three subcultures.
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Endotoxin ContentNMT 5EU/ml
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SolubilityClear solution at 15.8 gms/L.
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UsesLaboratory chemicals, Manufacture of substances
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Personal ProtectionUse personnel protective equipment. Wear disposable gloves, dust mask and eye protection. Avoid dust formation.
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Toxicological InformationNA
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OrderingTo order Nutrient Mixture F-10 Ham With L-Glutamine and 25mM HEPES buffer Without Sodium bicarbonate, please use the Cat. Nr.AT083-5X10X1L and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
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TipsOur specialists recommend you to follow carefully the pre-registered instructions for Nutrient Mixture F-10 Ham With L-Glutamine and 25mM HEPES buffer Without Sodium bicarbonate
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Additional descriptionBuffering solutions are useful to keep the pH range sable when using this reagent of Himedia.
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Buffer25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.
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Gene target
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Gene symbolF10, F10-AS1, HLA-F, H3C11, IGHV1-69-2, SNAR-F, PIRC49, KLRC4, SNORD115-10
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Short nameNutrient Mixture F-10 Ham With L-Glutamine 25mM Without Sodium bicarbonate
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Alternative nameNutrient Mixture F-10 Ham including L-Glutamine and 25mM HEPES solution Without Sodium bicarbonate
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Gene info
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Identity
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Gene
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Long gene namecoagulation factor X
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Classification
- Gla domain containing
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameF10 antisense RNA 1
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Synonyms gene name
- F10 antisense RNA 1 (non-protein coding)
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Locus
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Discovery year2011-05-09
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Classification
- Antisense RNAs
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemajor histocompatibility complex, class I, F
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GenBank acession
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Locus
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Discovery year1990-09-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- C1-set domain containing
- Histocompatibility complex
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameH3 clustered histone 11
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Synonyms gene
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Synonyms gene name
- H3 histone family, member F
- histone 1, H3i
- histone cluster 1, H3i
- histone cluster 1 H3 family member i
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-08-27
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- H3 histones
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 1-69-2
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Synonyms gene
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Synonyms gene name
- immunoglobulin heavy variable 1-F (provisional)
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesmall NF90 (ILF3) associated RNA F
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Synonyms gene name
- small nuclear ILF3/NF90-associated RNA F
- small ILF3/NF90-associated RNA F
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GenBank acession
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Locus
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Discovery year2008-06-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small NF90 (ILF3) associated RNAs
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 49
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namekiller cell lectin like receptor C4
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Synonyms gene name
- killer cell lectin-like receptor subfamily C, member 4
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Synonyms
-
GenBank acession
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Locus
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Discovery year1998-09-03
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Killer cell lectin like receptors
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 115-10
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box