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Description
NCTC 109 Medium, With L-Glutamine Without Sodium bicarbonate, is an off-white to creamish white, homogenous powder. It was originally formulated to establish and maintain a strain of mouse cells, L929. It has been shown to support growth of fibroblast-like and epithelial- like cells of both normal and malignant origin from mice, hamsters, monkeys and humans.
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Shelf life
Use before expiry date given on the product label.
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Storage
Store at 2-8°C away from bright light
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Appearance
Off-white to Creamish white, homogenous powder.
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PH without Sodium Bicarbonate
3.00 -3.60
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PH with Sodium Bicarbonate
6.80 -7.40
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Osmolality without Sodium Bicarbonate
230.00 -270.00
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Osmolality with Sodium Bicarbonate
280.00 -320.00
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Cultural Response
The growth promotion capacity of the medium is assessed qualitatively by analyzing the cells for the morphology and quantitatively by estimating the cell counts and comparing it with a control medium through minimum three subcultures.
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Endotoxin Content
NMT 5EU/ml
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Solubility
Clear solution at 9.7 gms/L.
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Uses
Laboratory chemicals, Manufacture of substances
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Personal Protection
Use personnel protective equipment. Wear disposable gloves, dust mask and eye protection. Avoid dust formation.
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Toxicological Information
Acute Effects : May be harmful if swallowed. Exposure can cause : Stomach pains, vomiting, diarrhoea. Prolonged or repeated exposure may cause allergic reactions in certain sensitive individuals. Prolonged or repeated exposure may cause allergic reactions in certain sensitive individuals.
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Ordering
To order NCTC 109 Medium With L-Glutamine Without Sodium bicarbonate, please use the Cat. Nr.AT138-20X1L and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
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Tips
Our specialists recommend you to follow carefully the pre-registered instructions for NCTC 109 Medium With L-Glutamine Without Sodium bicarbonate
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Additional description
There are two major types of growth media those used for cell culture , which use specific cell types derived from plants or animals, and microbiological culture, which are used for growing microorganisms, such as bacteria or yeast. The most common growth media for microorganisms are nutrient broths and agar plates with antibiotics; specialized media are sometimes required for microorganism and cell culture growth.
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Group
media
