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Description
RPMI 1640, With L Glutamine Without Sodium bicarbonate, is an off-white to creamish white, homogenous powder. RPMI were developed for the culture of human normal and neoplastic cells in vitro. RPMI 1640 is the most commonly used medium in the series. It was specifically designed to support the growth of human lymphoblastoid cells in suspension culture. Now the medium is extensively used for a wide range of anchorage dependant cell lines. It is also known to support growth of cells in the absence of serum.
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Shelf life
Use before expiry date given on the product label.
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Storage
Store at 2-8°C away from bright light
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Appearance
Off-white to Creamish white, homogenous powder.
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PH without Sodium Bicarbonate
7.20 -7.80
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PH with Sodium Bicarbonate
7.60 -8.20
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Osmolality without Sodium Bicarbonate
230.00 -270.00
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Osmolality with Sodium Bicarbonate
230.00 -270.00
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Cultural Response
The growth promotion capacity of the medium is assessed qualitatively by analyzing the cells for the morphology and quantitatively by estimating the cell counts and comparing it with a control medium through minimum three subcultures.
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Endotoxin Content
NMT 5EU/ml
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Solubility
Clear solution at 10.4 gms/L.
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Uses
Laboratory chemicals, Manufacture of substances
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Personal Protection
Use personnel protective equipment. Wear disposable gloves, dust mask and eye protection. Avoid dust formation.
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Toxicological Information
NA
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Ordering
To order RPMI 1640 With L Glutamine Without Sodium bicarbonate, please use the Cat. Nr.AT028-10X5L and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
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Tips
Our specialists recommend you to follow carefully the pre-registered instructions for RPMI 1640 With L Glutamine Without Sodium bicarbonate