Methylene Blue (Loeffler’s)

Description

Methylene Blue (Loeffler’s) is a readymade staining solution reported to be used in metachromatic staining
Other product
Application

For microbiological / pathological laboratories, hospitals, research settings and educational institutes. These stains augment tissue morphology, demonstrate important cellular components, aid in the evaluation of diseases, and identifies pathogenic organisms.
Classification

Acid Fast Stains
Composition

Methylene blue 0.30 gm Ethyl alcohol, 95% 30.0 ml Distilled water 100.0 ml
Appearance

Dark blue coloured solution
Clarity

Clear without any particles.
Microscopic Examination

Metachromatic staining is carried out where Methylene Blue is used as one of the stains and staining characteristic of organisms is observed under microscope by using oil immersion lens.
Results

Metachromatic granules : Deep blue, Cytoplasm : Pale blue
Storage

Store below 30°C in tightly closed container and away from bright light.
Shelf Life

Use before expiry date on label.
Technical Data
Data Sheet
Properties

Blue has a wavelength of around 480 nm and will make your sample visible. If your sample is too concentrated you can dissolve it in water.
Conjugation

Blue Substrate