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Description
Neisser’s Methylene Blue is a readymade staining solution reported to be used in metachromatic staining
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Application
For microbiological / pathological laboratories, hospitals, research settings and educational institutes. These stains augment tissue morphology, demonstrate important cellular components, aid in the evaluation of diseases, and identifies pathogenic organisms.
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Classification
Metachromatic Stains
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Composition
Methylene blue 1.000 gm Ethyl alcohol, 95% 50.000 ml Glacial acetic acid 50.000 ml Distilled water 100.000 ml
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Appearance
Blue coloured, solution.
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Clarity
Clear without any particles.
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Microscopic Examination
Metachromatic staining is carried out and staining characteristic of organism is observed under microscope by using oil immersion lens.
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Results
Metachromatic granules : deep blue, The Organisms : pink coloured.
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Storage
Store below 30°C in tightly closed container and away from bright light.
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Shelf Life
Use before expiry date on label.
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Properties
Blue has a wavelength of around 480 nm and will make your sample visible. If your sample is too concentrated you can dissolve it in water.
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Conjugation
Blue Substrate
