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Description
Medium 199, With Hank's salts Without L-Glutamine and Sodium bicarbonate, is a red colored, clear solution. This medium was formulated specifically for nutritional studies on primary chick embryo fibroblasts in the absence of any additives. It is presently used for the maintenance of non-transformed cells, vaccine and virus production and primary explants of epithelial cells.
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Shelf life
18 months
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Storage
Store at 2-8°C away from bright light
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Appearance
Red colored, clear solution
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PH
4.20 - 4.40
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Osmolality in mOsm Kg H2O
NA
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Sterility
No bacterial or fungal growth is observed after 14 days of incubation, as per USP specification.
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Cultural Response
The growth promotion capacity of the medium is assessed qualitatively by analyzing the cells for the morphology and quantitatively by estimating the cell counts and comparing it with a control medium.
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Endotoxin Content
NMT 1EU/ml
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Uses
Laboratory chemicals, Manufacture of substances
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Personal Protection
Use personnel protective equipment. Wear disposable gloves, dust mask and eye protection. Avoid dust formation.
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Toxicological Information
NA
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Ordering
To order Medium 199 With Hank's salts Without L-Glutamine and Sodium bicarbonate, please use the Cat. Nr.AL192-30X500ML and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
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Tips
Our specialists recommend you to follow carefully the pre-registered instructions for Medium 199 With Hank's salts Without L-Glutamine and Sodium bicarbonate
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Additional description
There are two major types of growth media those used for cell culture , which use specific cell types derived from plants or animals, and microbiological culture, which are used for growing microorganisms, such as bacteria or yeast. The most common growth media for microorganisms are nutrient broths and agar plates with antibiotics; specialized media are sometimes required for microorganism and cell culture growth.
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Group
media
