Medium 199 With Earle's salts and L-Glutamine Without Sodium bicarbonate

• Catalog number
  AT014-2X50L
• Price
  Please ask
• Size
  2X50L

• Description
  Medium 199, With Earle's salts and L-Glutamine Without Sodium bicarbonate, is an off-white to creamish white, homogenous powder. This medium was formulated specifically for nutritional studies on primary chick embryo fibroblasts in the absence of any additives. It is presently used for the maintenance of non-transformed cells, vaccine and virus production and primary explants of epithelial cells.
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• Shelf life
  Use before expiry date given on the product label.
• Storage
  Store at 2-8°C away from bright light
• Appearance
  Off-white to Creamish white, homogenous powder.
• PH without Sodium Bicarbonate
  4.40 -5.00
• PH with Sodium Bicarbonate
  7.20 -7.80
• Osmolality without Sodium Bicarbonate
  230.00 -270.00
• Osmolality with Sodium Bicarbonate
  280.00 -320.00
• Cultural Response
  The growth promotion capacity of the medium is assessed qualitatively by analyzing the cells for the morphology and quantitatively by estimating the cell counts and comparing it with a control medium through minimum three subcultures.
• Endotoxin Content
  NMT 5EU/ml
• Solubility
  Clear solution at 9.5 gms/L.
• Uses
  Laboratory chemicals, Manufacture of substances
• Personal Protection
  Use personnel protective equipment. Wear disposable gloves, dust mask and eye protection. Avoid dust formation.
• Toxicological Information
  NA
• Technical Data
• Data sheet
• Ordering
  To order Medium 199 With Earle's salts and L-Glutamine Without Sodium bicarbonate, please use the Cat. Nr.AT014-2X50L and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
• Tips
  Our specialists recommend you to follow carefully the pre-registered instructions for Medium 199 With Earle's salts and L-Glutamine Without Sodium bicarbonate
• Additional description
  There are two major types of growth media those used for cell culture , which use specific cell types derived from plants or animals, and microbiological culture, which are used for growing microorganisms, such as bacteria or yeast. The most common growth media for microorganisms are nutrient broths and agar plates with antibiotics; specialized media are sometimes required for microorganism and cell culture growth.
• Group
  media

• Gene target
  199   With   Earle's   salts   L-Glutamine   Without   Sodium   bicarbonate  
• Short name
  Medium 199 With Earle's salts L-Glutamine Without Sodium bicarbonate
• Technique
  Medium, cell culture Media
• Alternative name
  Medium 199 including Earle's salts and L-Glutamine Without Sodium bicarbonate
• Alternative technique
  cellculture
• Virus
  hhv

MeSH Data

• Name
  In Vitro Techniques
• Concept
  Scope note: Methods to study reactions or processes taking place in an artificial environment outside the living organism.
• Tree numbers
  • E05.481
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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