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Description
M-Azide Broth Base is used to cultivate and enumerate Enterococci from water samples by membrane filter technique.
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Alternative Name
NA
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Type
Dehydrated Culture Media
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Origin
Animal
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Classification
Bacterial
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Composition g L
Tryptose 40.000 Yeast extract 10.000 Dextrose 2.000 Saccharose 100.000 Dipotassium phosphate 4.000 Sodium azide 0.400 Final pH ( at 25°C) 7.1±0.2
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Quantity required per litre
156,40 g
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Appearance
Cream to yellow homogeneous free flowing powder
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Colour and Clarity of prepared medium
Light yellow coloured clear solution without any precipitate
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Cultural response
Cultural characteristics observed after an incubation at 35-37°C for 48 hours with added 1% 2,3,5 Triphenyl Tetrazolium Chloride
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Storage
Store below 30°C in tightly closed container and the prepared medium at 2-8°C.
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Shelf Life
Use before expiry date on the label.
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Additional description
Sodium azide NaN3 is often used to prevent antibody degradation in 0,02% W/V concentrations. Himedia supplies azide free antibodies on request.
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Properties
º C. The broth is a modified culture medium for culturing isolated or enriched bacteria, yeasts and micro organisms.The buffered pH and low salt content of these media broths will ensure a perfect growth of the pathogens to be detected. The broth or culture medium is supplied in 500gr and 5 x 500gr quntities by Himedia. The broth's MSDS certificate is supplied for appoval of our broth's batch quality. The screening and selective enrichment of these pathogens and bacteriacan also be obtained by other broths and selective culture media supplements.
