Kohn Two Tube Medium No.2

Description

Kohn Two Tube Medium No.2 is employed to identify members of Enterobacteriaceae on the bas is of the fermentation of sucrose and salicin, motility, and also the production of H2S and indole.
Other Product
Alternative Name

NA
Type

Dehydrated Culture Media
Origin

Animal
Classification

Bacterial
Composition g L

Peptic digest of animal tissue 10.000 Casein enzymic hydrolysate 10.000 Sucrose 10.000 Salicin 10.000 Sodium chloride 5.000 Sodium thiosulphate 0.016 Disodium hydrogen orthophosphate 0.090 Bromothymol blue 0.020 Agar 3.000 Final pH ( at 25°C) 7.4±0.2
Quantity required per litre

48,13 g
Appearance

Cream to yellow homogeneous free flowing powder
Colour and Clarity of prepared medium

Green coloured, clear to slightly opalescent gel forms in tubes as butts
Cultural response

Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours
Storage

Store below 30°C in tightly closed container and the prepared medium at 2-8°C.
Shelf Life

Use before expiry date on the label.
Technical Data
Data Sheet
Additional description

There are two major types of growth media those used for cell culture , which use specific cell types derived from plants or animals, and microbiological culture, which are used for growing microorganisms, such as bacteria or yeast. The most common growth media for microorganisms are nutrient broths and agar plates with antibiotics; specialized media are sometimes required for microorganism and cell culture growth.
Group

media

Name

In Vitro Techniques
Concept

Scope note: Methods to study reactions or processes taking place in an artificial environment outside the living organism.
Tree numbers
- E05.481
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data