Kohn Two Tube Medium No.1 Base

Description

Kohn Two Tube Medium No.1 Base is employed to identify Enterobacteriaceae on the bas is of dextrose, mannitol fermentation and also urease production.
Other Product
Alternative Name

NA
Type

Dehydrated Culture Media
Origin

Animal
Classification

Bacterial
Composition g L

Peptic digest of animal tissue 15.000 Beef extract 2.000 Yeast extract 2.000 Dextrose 1.000 Mannitol 10.000 Phenol red 0.050 Agar 16.000 Final pH ( at 25°C) 7.2±0.2
Quantity required per litre

46,05 g
Appearance

Light yellow to light pink homogeneous free flowing powder
Colour and Clarity of prepared medium

Pink coloured, clear to slightly opalescent gel forms in tubes as slants with a generous butt
Cultural response

Cultural characteristics observed with added sterile 40% w/v Urea Solution (FD048) after an incubation at 35-37°C for 18-24 hours
Storage

Store below 30°C in tightly closed container and the prepared medium at 2-8°C
Shelf Life

Use before expiry date on the label.
Technical Data
Data Sheet
Additional description

There are two major types of growth media those used for cell culture , which use specific cell types derived from plants or animals, and microbiological culture, which are used for growing microorganisms, such as bacteria or yeast. The most common growth media for microorganisms are nutrient broths and agar plates with antibiotics; specialized media are sometimes required for microorganism and cell culture growth.
Group

media

Name

In Vitro Techniques
Concept

Scope note: Methods to study reactions or processes taking place in an artificial environment outside the living organism.
Tree numbers
- E05.481
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data