Hugh Leifson Medium

Description

Hugh Leifson Medium is used for distinguishing anaerobic from aerobic breakdown of carbohydrate (glucose)
Other Product
Alternative Name

NA
Type

Dehydrated Culture Media
Origin

Animal
Classification

Bacterial
Composition g L

Peptone 2.000 Sodium chloride 5.000 Dipotassium phosphate 0.300 Glucose (Dextrose) 10.000 Bromothymol blue 0.030 Agar 3.000 Final pH ( at 25°C) 7.1±0.2
Quantity required per litre

20,33 g
Appearance

Light yellow to bluish green homogeneous free flowing powder
Colour and Clarity of prepared medium

Greenish blue coloured, clear to slightly opalescent gel forms in tubes as butts
Cultural response

Cultural characteristics observed after an incubation at 35-37°C for 18-48 hours.
Storage

Store between 10-30°C in a tightly closed container and the prepared medium at 2-8°C
Shelf Life

Use before expiry date on the label.
Technical Data
Data Sheet
Additional description

There are two major types of growth media those used for cell culture , which use specific cell types derived from plants or animals, and microbiological culture, which are used for growing microorganisms, such as bacteria or yeast. The most common growth media for microorganisms are nutrient broths and agar plates with antibiotics; specialized media are sometimes required for microorganism and cell culture growth.
Group

media

Name

Primary Cell Culture
Concept

Scope note: The initial culturing of cells derived directly from fresh TISSUES.
Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data