Hugh Leifson Glucose Medium

Description

Hugh Leifson Glucose Medium is used to differentiateStaphylococci from Micrococci on the bas is of glucose fermentation anaerobically.
Other Product
Alternative Name

NA
Type

Dehydrated Culture Media
Origin

Animal
Classification

Bacterial
Composition g L

Peptic digest of animal tissue 2.000 Yeast extract 0.500 Sodium chloride 30.000 Glucose 10.000 Bromocresol purple 0.015 Agar 3.000 Final pH ( at 25°C) 7.4±0.2
Quantity required per litre

45,52 g
Appearance

Light yellow to bluish grey homogeneous free flowing powder
Colour and Clarity of prepared medium

Purple coloured, clear to slightly opalescent gel forms in tubes as butts
Cultural response

Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours .
Storage

Store below 30°C in tightly closed container and the prepared medium at 2-8°C.
Shelf Life

Use before expiry date on the label.
Technical Data
Data Sheet
Additional description

There are two major types of growth media those used for cell culture , which use specific cell types derived from plants or animals, and microbiological culture, which are used for growing microorganisms, such as bacteria or yeast. The most common growth media for microorganisms are nutrient broths and agar plates with antibiotics; specialized media are sometimes required for microorganism and cell culture growth.
Group

media

Name

Primary Cell Culture
Concept

Scope note: The initial culturing of cells derived directly from fresh TISSUES.
Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data